Bioconjugation by copper(I)-catalyzed azide-alkyne [3 + 2] cycloaddition.

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Summary

The copper-catalyzed cycloaddition reaction between azides and alkynes functions efficiently in aqueous solution in the presence of a tris(triazolyl)amine ligand to make rapid and reliable covalent connections to micromolar concentrations of protein decorated with either of the reactive moieties.

Type
article
Published
2003-02-22
Cited by
1,451
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Keywords

Chemistry, Bioconjugation, Cycloaddition, Azide, Alkyne

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