Covalent protein-oligonucleotide conjugates by copper-free click reaction
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Summary
Cu-free click bioconjugation of proteins to ODNs is a simple and versatile alternative to Cu-catalyzed click methods, and the yields and reaction times are comparable to those previously reported for copper-catalyzed azide-alkyne [3+2] cycloaddition (CuAAC) bioconjugation, but require no catalyst.
- Type
- article
- Published
- 2012-05-17
- Cited by
- 42
- References
- 60
- Access
- Open access
- OpenAlex
- https://openalex.org/W1996012633
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:24130253
Keywords
Bioconjugation, Chemistry, Click chemistry, Azide, Cycloaddition
References
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- Single-molecule FRET studies of important intermediates in the nucleocapsid-protein-chaperoned minus-strand transfer step in HIV-1 reverse transcription.
- Improved green fluorescence
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- Surface functionalization using catalyst-free azide-alkyne cycloaddition.
- The developments of semisynthetic DNA-protein conjugates.
- A Facile Method for Reversibly Linking a Recombinant Protein to DNA
- Copper-free click chemistry for highly luminescent quantum dot conjugates: application to in vivo metabolic imaging.
- Visualizing metabolically labeled glycoconjugates of living cells by copper-free and fast huisgen cycloadditions.
- Thiol-based, site-specific and covalent immobilization of biomolecules for single-molecule experiments
- Copper-free click chemistry for dynamic in vivo imaging
- Oxidative Nucleobase Modifications Leading to Strand Scission.
- Covalent protein-oligonucleotide conjugates for efficient delivery of antisense molecules.
- Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates.
- Cu-free click cycloaddition reactions in chemical biology
- DNA-Directed immobilization: efficient, reversible, and site-selective surface binding of proteins by means of covalent DNA-streptavidin conjugates.
- Purification of prenylated proteins by affinity chromatography on cyclodextrin-modified agarose
- Peptide-Functionalized Click Hydrogels with Independently Tunable Mechanics and Chemical Functionality for 3D Cell Culture
- Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.
- Synthesis of protein-nucleic acid conjugates by expressed protein ligation.
Cited by
- Development of a simple method for protein conjugation by copper-free click reaction and its application to antibody-free Western blot analysis.
- Photocontrolled micellar aggregation of amphiphilic DNA-azobenzene conjugates.
- Oriented immobilization of proteins on solid supports for use in biosensors and biochips: a review
- Chemical and enzymatic tools to study proteins in their native cellular environment
- Design of a Ribosyltriazole‐Annulated Cyclooctyne for Oligonucleotide Labeling by Strain‐Promoted Alkyne–Azide Cycloaddition
- Synthesis of Site-Specific DNA–Protein Conjugates and Their Effects on DNA Replication
- DNA-associated click chemistry
- Advances in DNA-directed immobilization.
- Chemoenzymatic Labeling of Proteins: Techniques and Approaches
- Oligonucleotide Tagging for Copper-Free Click Conjugation
- Chemical approaches for the construction of multi-enzyme reaction systems.
- FRET-based cyanine probes for monitoring ligation reactions and their applications to mechanistic studies and catalyst screening.
- Specific immobilization of biotinylated fusion proteins NGF and Sema3A utilizing a photo-cross-linkable diazirine compound for controlling neurite extension.
- Protein Prenylation: Enzymes, Therapeutics, and Biotechnology Applications
- Development and Implementation of Multi-Cued Guidance Strategies for Axonal Regeneration
- In situ protein detection with enhanced specificity using DNA-conjugated antibodies and proximity ligation
- Primary Amine-Clustered DNA Aptamer for DNA-Protein Conjugation Catalyzed by Microbial Transglutaminase.
- Design and synthesis of ortho-phthalaldehyde phosphoramidite for single-step, rapid, efficient and chemoselective coupling of DNA with proteins under physiological conditions.
- Recent progress in enzymatic protein labelling techniques and their applications
- Covalent "Click Chemistry"-Based Attachment of DNA onto Solid Phase Enables Iterative Molecular Analysis.
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