Extended application of RP-HPLC for characterization of human hemoglobin variants.
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Summary
The different steps for the structural study of a human hemoglobin, from protein isolation to amino acid analysis, can be achieved using as the only method reversed phase high performance liquid chromatography.
- Type
- article
- Published
- 1987-01-15
- Cited by
- 35
- References
- 15
- OpenAlex
- https://openalex.org/W2014276704
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:20126931
Keywords
High-performance liquid chromatography, Chromatography, Hemoglobin, Chemistry, Hydrolysis
References
- Cleavage at glutamic acid with staphylococcal protease.
- Immobilized pH gradients and reversed-phase high-performance liquid chromatography: a strategy for characterization of haemoglobin variants with electrophoretic mobility identical to that of Hb A. The case of Hb San Diego.
- Tryptic cleavage at cysteinyl peptide bonds.
- High-performance liquid chromatography of biopolymers.
- Japanese Haemoglobin Variant
- Hemoglobin Grady: the first example of a variant with elongated chains due to an insertion of residues.
- Rapid high-performance liquid chromatographic method for the separation of the three types of γ-chain of human fetal haemoglobin
- Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate.
- PROTEIN COAGULATION AND ITS REVERSAL
- Man's Hæmoglobins
Cited by
- Capillary zone electrophoresis: an additional technique for the identification of hemoglobin variants.
- Electrophoretically silent hemoglobin mutants as revealed by isoelectric focusing in immobilized pH gradients
- Cleavage by protease from Staphylococcus aureus V8: an improvement in the sequence analysis of human hemoglobin variants.
- Improvement of oxygen-carrying properties of human hemoglobin by chemical modification with a benzene hexacarboxylate-monosubstituted polyoxyethylene
- Hb Puttelange [beta 140(H19)Ala-->Val] in an Italian man with polycythemia.
- MPSA short communications
- Hb Saint Nazaire (β103[G5]Phe→Ile): A new example of polycythemia due to a hemoglobin variant with increased oxygen affinity
- Two new cases of Hb Denmark Hill [alpha 95(G2)Pro----Ala] discovered in south-west France: electrophoretical and chromatographical considerations.
- A new hemoglobin variant found during investigations of diabetes mellitus: Hb Pavie [α 135 (H18) Val → Glu]
- Isoelectric focusing of tryptic peptides from hemoglobin subunits by immobilized pH gradients.
- Primary structure of the cytoplasmic domain of human erythrocyte protein band 3. Comparison with its sequence in the mouse.
- Two new human hemoglobin variants caused by unusual mutational events: Hb Zaïre contains a five residue repetition within the α-chain and Hb Duino has two residues substituted in the β-chain
- Hemoglobin Nouakchott [alpha 114(GH2)Pro----Leu]: a new hemoglobin variant displaying an unusual increase in hydrophobicity.
- Hemoglobin Calais [beta 76 (E20) Ala----Pro]: a hemoglobin variant with decreased intrinsic oxygen affinity.
- HB Al-Ain Abu Dhabi [alpha 18(A16)Gly----Asp]: a new hemoglobin variant discovered in an Emiratee family.
- HPLC-methods in the clinical-chemical laboratory of the department of dermatology of the University of Bonn
- Hb Luxembourg [alpha 24(B5) Tyr----His]: a new unstable variant.
- Hemoglobin Thionville. An alpha-chain variant with a substitution of a glutamate for valine at NA-1 and having an acetylated methionine NH2 terminus.
- Structure of the EF corner favors deamidation of asparaginyl residues in hemoglobin: the example of Hb La Roche-sur-Yon [beta 81 (EF5) Leu----His].
- Hemoglobin redondo [β 92(F8) His → Asn]: An unstable hemoglobin variant associated with heme loss which occurs in two forms
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