Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces
Explore this paper's citation graph
Summary
16S rRNA gene-targeted group-specific primers were designed and validated for specific detection and quantification of the Clostridium leptum subgroup and the Atopobium cluster and revealed that the composition of the flora of each volunteer remained stable throughout the test period.
- Type
- article
- Published
- 2004-12-01
- Cited by
- 691
- References
- 36
- Access
- Open access
- OpenAlex
- https://openalex.org/W2014216380
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:1506331
Keywords
Bacteroides fragilis, Prevotella, Feces, Biology, Bacteroides
References
- Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples
- Human fecal flora: the normal flora of 20 Japanese-Hawaiians.
- Intestinal floras of populations that have a high risk of colon cancer
- Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria.
- Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria
- Comparison of fecal microflora of elderly persons in rural and urban areas of Japan
- Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria
- Development of multi-color FISH method for analysis of seven Bifidobacterium species in human feces.
- Fecal Microbial Diversity in a Strict Vegetarian as Determined by Molecular Analysis and Cultivation
- Rapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S-23S rRNA intergenic spacer region and its flanking 23S rRNA.
- A new version of the RDP (Ribosomal Database Project)
- Phylogenetic Analysis of the Human Gut Microbiota Using 16S rDNA Clone Libraries and Strictly Anaerobic Culture‐Based Methods
- Rapid Detection and Quantification of Five Periodontopathic Bacteria by Real‐Time PCR
- Comparison of the Oral Bacterial Flora in Saliva from a Healthy Subject and Two Periodontitis Patients by Sequence Analysis of 16S rDNA Libraries
- Normal microflora: An introduction to microbes inhabiting the human body
- Quantification of Bacterial Groups within Human Fecal Flora by Oligonucleotide Probe Hybridization
- Rapid identification and quantification of Collinsella aerofaciens using PCR.
- Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human Feces
- The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
- PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples
Cited by
- Microbial ecology of the closed artificial ecosystem MELiSSA (Micro-Ecological Life Support System Alternative): reinventing and compartmentalizing the Earth's food and oxygen regeneration system for long-haul space exploration missions.
- Effect of Antibiotic Therapy on Human Fecal Microbiota and the Relation to the Development of Clostridium difficile
- Gastric acid reduction leads to an alteration in lower intestinal microflora.
- Medicinal lavender modulates the enteric microbiota to protect against Citrobacter rodentium-induced colitis.
- Bacteroides uniformis CECT 7771 Ameliorates Metabolic and Immunological Dysfunction in Mice with High-Fat-Diet Induced Obesity
- Biomarkers of chemotherapy-induced diarrhoea: a clinical study of intestinal microbiome alterations, inflammation and circulating matrix metalloproteinases
- Differences in gut microbiota composition between obese and lean children: a cross-sectional study
- Dietary supplementation of Bifidobacterium longum strain AH1206 increases its cecal abundance and elevates intestinal interleukin-10 expression in the neonatal piglet.
- Characterization of the Gut Microbiota of Papua New Guineans Using Reverse Transcription Quantitative PCR
- Iron, microbiota and colorectal cancer
- Consumption of an acid protease derived from Aspergillus oryzae causes bifidogenic effect in rats.
- Effects of ewe’s milk yogurt (whole and semi-skimmed) and cow’s milk yogurt on inflammation markers and gut microbiota of subjects with borderline-high plasma cholesterol levels: a crossover study
- Gender Difference and Dietary Supplemental Vitamin B6: Impact on Colon Luminal Environment.
- Changes in human faecal microbiota due to chemotherapy analyzed by TaqMan-PCR and PCR-DGGE
- [Development of quantitative PCR detection method with 16S rRNA gene-targeted genus- and species-specific primers for the analysis of human intestinal microflora and its application].
- Rapid detection of Enterobacter sakazakii using TaqMan real-time PCR assay.
- Shifts of fecal microbiota in immunosuppressed patients following chemotherapeutic treatment analyzed by TaqMan-PCR and PCR-DGGE with special focus on 454 sequencing
- Detection of Enteric Viruses in East Tennessee Public Ground Water Systems
- Gastrointestinal microbiota in type 2 diabetes
- The commensal microbiology of the gastrointestinal tract.
Related papers
- Superoxide dismutase in Bacteroides fragilis and related Bacteroides species
- Adherence of Bacteroides fragilis group species
- Heterologous expression of the Bacteroides ruminicola xylanase gene in Bacteroides fragilis and Bacteroides uniformis.
- Association of dietary patterns with the fecal microbiota in Korean adolescents
- Anti-bacteroides lipopolysaccharide IgG levels in healthy adults and sepsis patients.
- Effect of encapsulated Bacteroides asaccharolyticus and Bacteroides fragilis on the growth of aerobic and facultative bacteria in mixed infections.
- Interpreting Prevotella and Bacteroides as biomarkers of diet and lifestyle
- Activity of trospectomycin against Bacteroides fragilis and other Bacteroides species
- Investigation into the stability and culturability of Chinese enterotypes