Immunohistochemical detection of thrombospondin-1 in formalin-fixed, paraffin-embedded tissue.
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Summary
The results demonstrate that TSP can be reliably detected in paraffin-embedded tissue by immunohistochemical techniques that utilize AR with high-temperature microwave heating and a low-pH Tris-HCI buffer.
- Type
- article
- Published
- 1996-07-01
- Cited by
- 34
- References
- 27
- Access
- Open access
- OpenAlex
- https://openalex.org/W2013311810
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:30712836
Keywords
Immunohistochemistry, Antigen retrieval, Thrombospondin, Metastasis, Thrombospondin 1
References
- Thrombospondins: structure and regulation of expression
- Thrombospondin in human glomerulopathies. A marker of inflammation and early fibrosis.
- Modulation of keratinocyte motility. Correlation with production of extracellular matrix molecules in response to growth promoting and antiproliferative factors.
- The structure of human platelet thrombospondin.
- Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies.
- Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry
- Thrombospondin-induced attachment and spreading of human squamous carcinoma cells.
- Differential expression of SPARC and thrombospondin 1 in wound repair: immunolocalization and in situ hybridization.
- Control of angiogenesis in fibroblasts by p53 regulation of thrombospondin-1.
- Thrombospondin binding by human squamous carcinoma and melanoma cells: relationship to biological activity.
- The functions of thrombospondin and its involvement in physiology and pathophysiology.
- A tumor suppressor-dependent inhibitor of angiogenesis is immunologically and functionally indistinguishable from a fragment of thrombospondin.
- Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections.
- Colocalization of thrombospondin and syndecan during murine development
- Modulation of thrombospondin expression during differentiation of embryonal carcinoma cells
- Platelet thrombospondin mediates attachment and spreading of human melanoma cells
- Thrombospondin-induced tumor cell migration: haptotaxis and chemotaxis are mediated by different molecular domains
- Light microscopic immunolocation of thrombospondin in human tissues.
- Platelet thrombospondin modulates endothelial cell adhesion, motility, and growth: a potential angiogenesis regulatory factor
- Thrombospondin exerts an antiangiogenic effect on cord formation by endothelial cells in vitro.
Cited by
- Thrombospondin‐1, vascular endothelial growth factor expression and their relationship with p53 status in prostate cancer and benign prostatic hyperplasia
- Microscopy, Immunohistochemistry, and Antigen Retrieval Methods
- Integrin characterization in pulmonary bronchioles.
- Optimizing immunohistochemistry: antigen retrieval and signal amplification
- Alterations in tumour suppressor gene p53 correlate with inhibition of thrombospondin-1 gene expression in colon cancer cells
- Stromal thrombospondin-1 expression is a prognostic indicator and a new marker of invasiveness in intraductal papillary-mucinous neoplasm of the pancreas.
- Optimal Methods of Antigen Retrieval for Organic Anion Transporters in Cryosections of the Rat Kidney
- Thrombospondin-1 expression in bladder cancer: association with p53 alterations, tumor angiogenesis, and tumor progression.
- Thrombospondin 2 expression is correlated with inhibition of angiogenesis and metastasis of colon cancer
- The expression of thrombospondin-1 and p53 in clear cell renal cell carcinoma: its relationship to angiogenesis, cell proliferation and cancer specific survival.
- Molecular prognostication in bladder cancer--a current perspective.
- The Distribution of the Matricellular Protein Thrombospondin 2 in Tissues of Embryonic and Adult Mice
- Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections: standardization and development.
- Expresson of vascular endothelial growth factor, its receptors (FLT-1, KDR) and TSP-1 related to microvessel density and patient outcome in vertical growth phase melanomas.
- Molecular pathways in bladder cancer
- Standardization of immunohistochemistry based on antigen retrieval technique for routine formalin-fixed tissue sections
- Expression of thrombospondin-1 in pancreatic carcinoma: correlation with microvessel density
- Thrombospondin-1 expression in oral squamous cell carcinomas: correlations with tumor vascularity, clinicopathological features and survival.
- Detection of Integrins in Formalin-fixed, Paraffin-embedded Tissues
- Prognostic markers in bladder cancer: a contemporary review of the literature.
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