Searching for peptide ligands with an epitope library.
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Summary
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".
- Type
- article
- Published
- 1990-07-27
- Cited by
- 2,038
- References
- 15
- OpenAlex
- https://openalex.org/W2010649076
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:33919073
Keywords
Epitope, Phage display, Peptide library, Peptide, Peptide sequence
References
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- Preliminary crystallographic data and primary sequence for anti-peptide Fab' B13I2 and its complex with the C-helix peptide from myohemerythrin.
- Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
- Immunogenicity and epitope mapping of foreign sequences via genetically engineered filamentous phage.
- Random peptide libraries: a source of specific protein binding molecules.
- Immunological Specificity
- Filamentous phage assembly: morphogenetically defective mutants that do not kill the host.
- Antibody-selectable filamentous fd phage vectors: affinity purification of target genes.
- Asymmetric orientation of a phage coat protein in cytoplasmic membrane of Escherichia coli.
- Rapid and efficient site-specific mutagenesis without phenotypic selection.
- Conformations of single-stranded DNA and coat protein in fd bacteriophage as revealed by ultraviolet absorption spectroscopy.
- Influence of protein flexibility and peptide conformation on reactivity of monoclonal anti-peptide antibodies with a protein alpha-helix.
- On the specificity of antibodies.
- DNA sequencing with chain-terminating inhibitors.
- High efficiency transformation of E. coli by high voltage electroporation
Cited by
- Design, construction and function of a multicopy display vector using fusions to the major coat protein of bacteriophage M13.
- Analysis of the Glycyrrhizic Acid-Binding Sites with Phage Display
- Staphylococcal surface display in directed evolution
- Development of antibody-based strategies for the detection of mastitis and its causes in dairy cattle
- Immunogenic Properties of Peptides Mimicking a HIV-1 gp41 Epitope Recognized by Virus-Neutralizing Antibodies 2F5
- Membrane insertion defects caused by positive charges in the early mature region of protein pIII of filamentous phage fd can be corrected by prlA suppressors
- M13 bacteriophage displaying disulfide-constrained microproteins.
- General M13 phage display: M13 phage display in identification and characterization of protein-protein interactions.
- Phage display shot-gun cloning of ligand-binding domains of prokaryotic receptors approaches 100% correct clones.
- Identification of GIYWHHY as a novel peptide substrate for human p60c-src protein tyrosine kinase.
- Probing antibody-antigen interactions by mass spectrometry.
- Phage-displayed peptide targeting on the Puumala hantavirus neutralization site
- Shot-gun phage display mapping of two streptococcal cell-surface proteins.
- Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography.
- Recombinant epstein-barr virus proteins and anti-body-selected peptides: reagents for serodiagnosis and vaccination
- Epitope discovery using bacteriophage display: the minimum epitope of an anti-IRBP antibody.
- Prediction of the Immunodominant Epitope of the Pyruvate Dehydrogenase Complex E2 in Primary Biliary Cirrhosis Using Phage Display
- Construction of high-complexity combinatorial phage display peptide libraries.
- Identification of distinct antibody epitopes and mimotopes from a peptide array of 5520 randomly generated sequences.
- High Affinity Mimotope of the Polysaccharide Capsule of Cryptococcus neoformans Identified from an Evolutionary Phage Peptide Library1
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