Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion
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Summary
Peptide synthesis on cellulose using SPOT technology allows the parallel synthesis of large numbers of addressable peptides in small amounts, and the cost per peptide is less than 1% of peptides synthesized conventionally on resin.
- Type
- article
- Published
- 2007-05-24
- Cited by
- 284
- References
- 39
- OpenAlex
- https://openalex.org/W2010560388
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:32143600
Keywords
Peptide, Cellulose, Combinatorial chemistry, Cleavage (geology), Amino acid
References
- Principles and Applications
- Peptides: Chemistry and Biology
- Ribosome-display technology: applications for directed evolution of functional proteins.
- Oxidative Reactions of Hydrazines. IV. Elimination of Nitrogen from 1,1-Disubstituted-2-arenesulfonhydrazides1-4
- Cross-reactivity of T lymphocytes in infection and autoimmunity
- Peptide display on bacterial flagella: principles and applications.
- 9-Fluorenylmethoxycarbonyl amino-protecting group
- Use of luminescent bacteria for rapid screening and characterization of short cationic antimicrobial peptides synthesized on cellulose using peptide array technology
- Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases.
- Characterizing and optimizing protease/peptide inhibitor interactions, a new application for spot synthesis.
- Noninvasive continuous monitoring of solid-phase peptide synthesis by acid-base indicator.
- Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support
- SPOT synthesis: reliability of array-based measurement of peptide binding affinity.
- Phage display systems and their applications
- Solid Phase Peptide Synthesis
- Mapping protein-protein contact sites using cellulose-bound peptide scans
- Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids.
- Hitting the SPOT: small-molecule macroarrays advance combinatorial synthesis.
- Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery
- Combining SPOT synthesis and native peptide ligation to create large arrays of WW protein domains.
Cited by
- Synthesis of peptide arrays using SPOT-technology and the CelluSpots-method.
- Biophysical characterization of recombinant proteins: A key to higher structural genomics success
- Systematic antibody and antigen-based proteomic profiling with microarrays
- A Highly Scalable Peptide-Based Assay System for Proteomics
- Expediting SRM assay development for large-scale targeted proteomics experiments
- Short linear cationic antimicrobial peptides: screening, optimizing, and prediction.
- Peptide Microarrays to Interrogate the “Histone Code”
- Assessment of antimicrobial (host defense) peptides as anti-cancer agents.
- Peptide arrays for enzyme profiling.
- Peptide arrays with a chip.
- Construction and Screening of Custom Peptide Libraries Derived from Synthetic DNA Pools.
- Screening peptide array library for the identification of cancer cell-binding peptides.
- Identifying reactive peptides from phage-displayed libraries
- The spot technique: synthesis and screening of peptide macroarrays on cellulose membranes.
- Epitope mapping of antibodies towards human protein targets
- The role of protein kinases in DNA replication in Saccharomyces cerevisiae
- A biosynthetic approach to the discovery of novel bioactive peptides
- Synthetic peptides for the immunodiagnosis of hepatitis A virus infection.
- Modelling as a tool for increasing the specific productivity of single-chain antibody fragments from Pichia pastoris
- SpikeTides[trade][mdash]proteotypic peptides for large-scale MS-based proteomics
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