Synthesis of peptide arrays using SPOT-technology and the CelluSpots-method.
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Summary
This method allows the synthesis of low-cost peptide arrays containing around 900 large spots of addressable peptides on a cellulose sheet of 19 cm x 29 cm.
- Type
- review
- Published
- 2009-01-01
- Cited by
- 57
- References
- 46
- OpenAlex
- https://openalex.org/W17327725
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:32254082
Keywords
Cellulose, Peptide, Combinatorial chemistry, Chemistry, Nanotechnology
References
- Peptide Arrays on Membrane Supports
- Solid phase peptide synthesis — A practical approach
- Highly parallel nano-synthesis of cleavable peptide-dye conjugates on cellulose membranes
- Ribosome-display technology: applications for directed evolution of functional proteins.
- Molecular Basis for the Substrate Specificity of NIMA-related Kinase-6 (NEK6)
- Cross-reactivity of T lymphocytes in infection and autoimmunity
- Peptide display on bacterial flagella: principles and applications.
- Protein labeling and biotinylation of peptides during spot synthesis using biotin p‐nitrophenyl ester (biotin‐ONp)
- Spatially addressed synthesis of amino- and amino-oxy-substituted 1, 3,5-triazine arrays on polymeric membranes.
- Complete substitutional analysis of a sunflower trypsin inhibitor with different serine proteases.
- Efficient assembly of peptomers on continuous surfaces
- Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion
- Noninvasive continuous monitoring of solid-phase peptide synthesis by acid-base indicator.
- Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support
- An improved method for the synthesis of cellulose membrane-bound peptides with free C termini is useful for PDZ domain binding studies.
- SPOT synthesis: reliability of array-based measurement of peptide binding affinity.
- Phage display systems and their applications
- Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids.
- Hitting the SPOT: small-molecule macroarrays advance combinatorial synthesis.
- Stable attachment of the HMB-linker to continuous cellulose membranes for parallel solid phase spot synthesis
Cited by
- A Novel Multipeptide Microarray for the Specific and Sensitive Mapping of Linear IgE-Binding Epitopes of Food Allergens
- SPOTing Acetyl-Lysine Dependent Interactions
- The Privileged Chemical Space Predictor (PCSP): A computer program that identifies privileged chemical space from screens of modularly assembled chemical libraries
- mTOR modulates the antibody response to provide cross-protective immunity to lethal influenza infections
- High Throughput Screening Methods for Assessing Antibiofilm and Immunomodulatory Activities of Synthetic Peptides
- Specificity analysis of protein lysine methyltransferases using SPOT peptide arrays.
- Application of Celluspots peptide arrays for the analysis of the binding specificity of epigenetic reading domains to modified histone tails
- Chromatin methylation activity of Dnmt3a and Dnmt3a/3L is guided by interaction of the ADD domain with the histone H3 tail
- Challenges in Optimizing a Prostate Carcinoma Binding Peptide, Identified through the Phage Display Technology
- Binding of the Phage Display Derived Peptide CaIX-P1 on Human Colorectal Carcinoma Cells Correlates with the Expression of Carbonic Anhydrase IX
- Multivalent recognition of histone tails by the PHD-fingers of CHD5
- Age-Related Memory Impairment Is Associated with Disrupted Multivariate Epigenetic Coordination in the Hippocampus
- Detailed specificity analysis of antibodies binding to modified histone tails with peptide arrays
- Histone tails – ideal motifs for probing epigenetics through chemical biology approaches
- Sensing Immune Responses with Customized Peptide Microarrays
- The ATRX-ADD domain binds to H3 tail peptides and reads the combined methylation state of K4 and K9.
- Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes.
- Glycoprotein Interactions in the Assembly of Hantaviruses
- Specificity Analysis of Histone Modification-Specific Antibodies or Reading Domains on Histone Peptide Arrays.
- Peptides and Peptide Analogs to Inhibit Protein-Protein Interactions.
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