Cloning and expression of methicillin resistance from Staphylococcus epidermidis in Staphylococcus carnosus
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Summary
A 6.2-kilobase chromosomal DNA fragment from a methicillin-resistant Staphylococcus epidermidis strain was cloned into Staphyllococcus carnosus by using staphylitiscal plasmid pCA44 as the vector and was responsible for the synthesis of a low-affinity penicillin -binding protein (PBP), PBP 2'.
- Type
- article
- Published
- 1988-10-01
- Cited by
- 65
- References
- 30
- OpenAlex
- https://openalex.org/W2009605795
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:7207304
Keywords
Staphylococcus epidermidis, Microbiology, Biology, Plasmid, Staphylococcus aureus
References
- Genetic behavior of the methicillin resistance determinant in Staphylococcus aureus
- Identification of a Staphylococcus aureus transposon (Tn4291) that carries the methicillin resistance gene(s)
- Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus
- Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus
- Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA
- Insertional inactivation of staphylococcal methicillin resistance by Tn551
- Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli.
- Construction of Staphylococcus plasmid vector pCA43 conferring resistance to chloramphenicol, arsenate, arsenite and antimony.
- Methicillin-resistant strains of Staphylococcus aureus; presence of identical additional penicillin-binding protein in all strains examined
- Intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus.
- Effects of temperature, NaCl, and methicillin on penicillin-binding proteins, growth, peptidoglycan synthesis, and autolysis in methicillin-resistant Staphylococcus aureus
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
- A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
- Transduction of penicillinase production and methicillin resistance-enterotoxin B production in strains of Staphylococcus aureus.
- Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus
- Detection of specific sequences among DNA fragments separated by gel electrophoresis.
- Decreased affinity of a penicillin-binding protein for β-lactam antibiotics in a clinical isolate of Staphylococcus aureus resistant to methicillin
- Antibody used to identify penicillin‐binding protein 2' in methicillin‐resistant strains of Staphylococcus aureus (MRSA)
- A novel site on the chromosome of Staphylococcus aureus influencing the level of methicillin resistance: genetic mapping
- IS431, a staphylococcal insertion sequence-like element related to IS26 from Proteus vulgaris.
Cited by
- Identification of a methicillin-resistant strain of Staphylococcus caprae from a human clinical specimen
- The dissemination among Staphylococcus aureus of the Staphylococcal Chromosome Cassette mec (SCCmec) which confers multiresistance
- [Detection of mecA gene of methicillin resistant staphylococci from oral swabs by polymerase chain reaction].
- Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory
- Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products
- The Staphylococcus aureus mec determinant comprises an unusual cluster of direct repeats and codes for a gene product similar to the Escherichia coli sn-glycerophosphoryl diester phosphodiesterase
- Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction
- Problems and Dilemmas of Antimicrobial Resistance
- Southern hybridization analysis of the mecA deletion from methicillin-resistant Staphylococcus aureus.
- Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis.
- Restriction maps of the regions coding for methicillin and tobramycin resistances on chromosomal DNA in methicillin-resistant staphylococci
- New aspects of antimicrobial resistance and the resulting therapeutic dilemmas.
- The Crisis in Antibiotic Resistance
- Expression of resistance to methicillin.
- Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains
- Factors affecting methicillin resistance in Staphylococcus aureus.
- Genomic diversity of mec regulator genes in methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis
- Cloning and restriction analysis of DNA conferring new quinolone antimicrobial agent resistance from Staphylococcus aureus and other coagulase-negative Staphylococcus species.
- Molecular Evolution of MRSA
- Correlation between regulation of mecA transcription and expression of methicillin resistance in staphylococci
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