A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
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Summary
A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
- Type
- article
- Published
- 1974-07-01
- Cited by
- 7,366
- References
- 7
- Access
- Open access
- OpenAlex
- https://openalex.org/W2034277598
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:27417779
Keywords
Polyacrylamide, Polyacrylamide gel electrophoresis, Scintillation, Nucleic acid, Tritium
References
- Reduction of ribosomal RNA synthesis and ribosomal RNA genes in a mutant of Xenopus laevis which organizes only a partial nucleolus. II. The number of ribosomal RNA genes in animals of different nucleolar types.
- Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
- Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis.
- Chemical post-labeling methods for the base composition and sequence analysis of RNA.
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
- Tritium and Paper Chromatography
Cited by
- Similar polypeptide composition of fast-transported proteins in rat motor and sensory axons.
- Antibody to virion structural proteins in mammals bearing avian sarcoma virus-induced tumors.
- Fast axonal transport of labeled protein in sensory axons during regeneration.
- Cytotoxic T lymphocyte membrane components: an analysis of structures related to function.
- Synthesis of lens crystallins in Xenopus oocytes as determined by quantitative immunoprecipitation.
- Identification of mRNA in the slime mold Physarum polycephalum.
- Adenovirus type 2 early polypeptides immunoprecipitated by antisera to five lines of adenovirus-transformed rat cells
- JC human papovavirus replication in human amnion cells
- Protein synthesis in oocytes of Xenopus laevis is not regulated by the supply of messenger RNA.
- Evidence for a role of RNA in eukaryotic chromosome structure. Metabolically stable, small nuclear RNA species are covalently linked to chromosomal DNA in HeLa cells.
- Leukocyte surface origin of human alpha1-acid glycoprotein (orosomucoid)
- Quantitative aspects of mucus glycoprotein biosynthesis in rat gastric mucosa.
- Transcription of the influenza virus genome.
- Binding of Bacteriocin Clo DF13 to Clo DF13 Plasmid Deoxyribonucleic Acid In Vivo and In Vitro
- Cryptic form of mRNA in dormant Artemia salina cysts.
- Organization and slow axonal transport of cytoskeletal proteins under normal and regenerating conditions
- The axonally secreted cell adhesion molecule, axonin-1. Primary structure, immunoglobulin-like and fibronectin-type-III-like domains and glycosyl-phosphatidylinositol anchorage.
- Infection of terminally differentiated myotubes with Rous sarcoma virus: reduced synthesis of env and v-src proteins.
- Heat and cold shock protein synthesis in arctic and temperate strains of rhizobia
- Human recombinant C5a enhances lipopolysaccharide‐induced synthesis of interleukin‐6 by human monocytes
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- Feature
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