Variegated expression of a globin transgene correlates with chromatin accessibility but not methylation status.
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Summary
Surprisingly, the restrictive chromatin structure observed at silenced transgene loci did not correlate with increased methylation, with transgenes from both active and inactive loci appearing largely unmethylated following analysis with methylation-sensitive restriction enzymes and by sequencing PCR products derived from bisulphite-converted genomic DNA.
- Type
- article
- Published
- 1996-12-01
- Cited by
- 52
- References
- 66
- Access
- Open access
- OpenAlex
- https://openalex.org/W2007926746
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:18919538
Keywords
Biology, Transgene, Chromatin, DNA methylation, Methylation
References
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- alpha-thalassemia resulting from a negative chromosomal position effect.
- Genetic and functional studies on the thyroid hormone axis
- Stable expression of green fluorescent protein after liposomal transfection of K562 cells without selective growth conditions.
- Epitope-tagging Math5 and Pou4f2: new tools to study retinal ganglion cell development in the mouse
- Isolation of undifferentiated and early differentiating Type A spermatogonia from Pou5f1-GFP reporter mice
- Human HMG box transcription factor HBP1: a role in hCD2 LCR function
- Mouse muscle identity: The position‐dependent and fast fiber‐specific expression of a transgene in limb muscles is methylation‐independent and cell‐autonomous
- Position effects in mice carrying a lacZ transgene in cis with the beta-globin LCR can be explained by a graded model.
- Transgenic Analysis of the Stem Cell Leukemia +19 Stem Cell Enhancer in Adult and Embryonic Hematopoietic and Endothelial Cells
- CTCF Regulates Growth and Erythroid Differentiation of Human Myeloid Leukemia Cells*
- Transgenic mice with hematopoietic and lymphoid specific expression of Cre
- Reversal of the silencing of tetracycline‐controlled genes requires the coordinate action of distinctly acting transcription factors
- Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping
- Differential regulation of laminin b1 transgene expression in the neonatal and adult mouse brain.
- Sensitive flow cytometric analysis reveals a novel type of parent-of-origin effect in the mouse genome.
- Reactivation of heritably silenced gene expression in mice
- Postnatal liver growth and regeneration are independent of c-myc in a mouse model of conditional hepatic c-myc deletion
- Stochastic, stage-specific mechanisms account for the variegation of a human globin transgene.
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