Mechanistic investigation of the endo-α-N-acetylgalactosaminidase from Streptococcus pneumoniae R6
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Summary
A mechanistic investigation of the SpGH101 retaining α-glycoside hydrolase using a combination of synthetic and natural substrates is reported here and proposes D764 and E796 as the nucleophile and general acid/base residues respectively.
- Type
- article
- Published
- 2009-11-03
- Cited by
- 27
- References
- 32
- Access
- Open access
- OpenAlex
- https://openalex.org/W2003335852
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:30461393
Keywords
Chemistry, Glycan, Stereochemistry, Enzyme, Glycoside hydrolase
References
- Identification of the Catalytic Nucleophile of the Family 29 α-L-Fucosidase from Thermotoga maritima through Trapping of a Covalent Glycosyl-Enzyme Intermediate and Mutagenesis*
- Recruitment of both uniform and differential binding energy in enzymatic catalysis: xylanases from families 10 and 11.
- The PyMOL Molecular Graphics System
- Molecular cloning and expression of endo-beta-N-acetylglucosaminidase D, which acts on the core structure of complex type asparagine-linked oligosaccharides.
- Genome of the Bacterium Streptococcus pneumoniae Strain R6
- Mechanisms of cellulases and xylanases: a detailed kinetic study of the exo-beta-1,4-glycanase from Cellulomonas fimi.
- Identification of the acid/base catalyst in Agrobacterium faecalis beta-glucosidase by kinetic analysis of mutants.
- The Structural Basis for T-antigen Hydrolysis by Streptococcus pneumoniae
- Mechanistic analyses of catalysis in human pancreatic alpha-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids.
- A case for reverse protonation: identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum beta-xylosidase and detailed kinetic analysis of a site-directed mutant.
- Dissection of nucleophilic and acid-base catalysis in glycosidases.
- Identification and Molecular Cloning of a Novel Glycoside Hydrolase Family of Core 1 Type O-Glycan-specific Endo-α-N-acetylgalactosaminidase from Bifidobacterium longum*
- Glycosidase mechanisms: anatomy of a finely tuned catalyst.
- Crystallographic and mutational analyses of substrate recognition of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum.
- SWEET - WWW-based rapid 3D construction of oligo- and polysaccharides
- Complete Genome Sequence of a Virulent Isolate of Streptococcus pneumoniae
- The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics
- Relationship of sequence and structure to specificity in the alpha-amylase family of enzymes.
- Facile synthesis of 2,4-dinitrophenyl alpha-D-glycopyranosides as chromogenic substrates for alpha-glycosidases.
- Mutagenesis of glycosidases.
Cited by
- Synthesis of 2-acetamido-1,2-dideoxy-d-galacto-nojirimycin [DGJNAc] from d-glucuronolactone: the first sub-micromolar inhibitor of α-N-acetylgalactosaminidases
- Gh101 Family of Glycoside hydrolases: subfamily Structure and Evolutionary Connections with Other Families
- Exploring novel non-Leloir β-glucosyltransferases from proteobacteria for modifying linear (β1->3)-linked gluco-oligosaccharide chains.
- Glycan microarrays for decoding the glycome
- Scalable syntheses of both enantiomers of DNJNAc and DGJNAc from glucuronolactone: the effect of N-alkylation on hexosaminidase inhibition.
- Pneumococcal surface proteins: when the whole is greater than the sum of its parts.
- A novel fluorescent assay for T-synthase activity.
- Structural Analysis of a Family 101 Glycoside Hydrolase in Complex with Carbohydrates Reveals Insights into Its Mechanism*
- Mechanistic studies of retaining α-glycosidases
- Defining the role of pneumococcal neuraminidases and O-glycosidase in pneumococcal haemolytic uraemic syndrome.
- Carbohydrate processing by bacterial pathogens: structural and functional analyses of glycoside hydrolases.
- Multiple exo-glycosidases in human serum as detected with the substrate DNP-α-GalNAc. II. Three α-N-acetylgalactosaminidase-like activities in the pH 5 to 8 region
- Multiple exo-glycosidases in human serum as detected with the substrate DNP-α-GalNAc. I. A new assay for lysosomal α-N-acetylgalactosaminidase
- Glycan‐metabolizing enzymes in microbe–host interactions: the Streptococcus pneumoniae paradigm
- Biochemical characterization of the endo-α-N-acetylgalactosaminidase pool of the human gut symbiont Tyzzerella nexilis.
- Discovery and Development of Promiscuous O-Glycan Hydrolases for Removal of Intact Sialyl T-Antigen.
- Identification of a Catalytic Nucleophile-Activating Network in the endo-α-N-Acetylgalactosaminidase of Family GH101.
- A Synthetic Gene Library Yields a Previously Unknown Glycoside Phosphorylase That Degrades and Assembles Poly-β-1,3-GlcNAc, Completing the Suite of β-Linked GlcNAc Polysaccharides
- Engineering Bifidobacterium longum Endo-α-N-acetylgalactosaminidase for Neu5Acα2-3Galβ1-3GalNAc reactivity on Fetuin.
- Human Saliva Modifies Growth, Biofilm Architecture and Competitive Behaviors of Oral Streptococci
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