Identification of the Catalytic Nucleophile of the Family 29 α-L-Fucosidase from Thermotoga maritima through Trapping of a Covalent Glycosyl-Enzyme Intermediate and Mutagenesis*
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Summary
The first direct identification of the catalytic nucleophile of an α-l-fucosidase is presented, a key step in the understanding of these important enzymes.
- Type
- article
- Published
- 2003-11-28
- Cited by
- 72
- References
- 39
- Access
- Open access
- OpenAlex
- https://openalex.org/W2089193762
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:45799281
Keywords
Thermotoga maritima, Glycoconjugate, Biochemistry, Fucosidase, Enzyme
References
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- 2-Deoxy-2-fluoro-D-glycosyl fluorides. A new class of specific mechanism-based glycosidase inhibitors.
- Identification of an archaeal alpha-L-fucosidase encoded by an interrupted gene. Production of a functional enzyme by mutations mimicking programmed -1 frameshifting.
- Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli.
- Identification of the catalytic nucleophile of the Family 31 alpha-glucosidase from Aspergillus niger via trapping of a 5-fluoroglycosyl-enzyme intermediate.
- Syntheses of 2-deoxy-2-fluoro mono- and oligo-saccharide glycosides from glycals and evaluation as glycosidase inhibitors.
- Adhesion receptors of the immune system
- Maltodextrin‐binding proteins from diverse bacteria and archaea are potent solubility enhancers
- Carbohydrate differentiation antigens: probable ligands for cell adhesion molecules.
- Involvement of adhesion molecules in metastasis of SW1990, human pancreatic cancer cells
- A convenient synthesis of 1,2-difluoro-1, 2-dideoxyhexoses using xenon fluoride
- Glycoprotein lysosomal storage disorders: alpha- and beta-mannosidosis, fucosidosis and alpha-N-acetylgalactosaminidase deficiency.
- Mechanistic consequences of mutation of active site carboxylates in a retaining beta-1,4-glycanase from Cellulomonas fimi.
- Mechanistic analyses of catalysis in human pancreatic alpha-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids.
- Spectrum of mutations in fucosidosis
- Mechanism of the family 1 beta-glucosidase from Streptomyces sp: catalytic residues and kinetic studies.
- Identification of Asp197 as the catalytic nucleophile in the family 38 α‐mannosidase from bovine kidney lysosomes
- Dissection of nucleophilic and acid-base catalysis in glycosidases.
- Leukocyte trafficking mediated by selectin-carbohydrate interactions
Cited by
- Structural and functional studies on secreted glycoside hydrolases produced by clostridium perfringens
- Probing the catalytic mechanism of bovine CD38/NAD+ glycohydrolase by site directed mutagenesis of key active site residues.
- Three dimensional structure of a bacterial α-l-fucosidase with a 5-membered iminocyclitol inhibitor.
- Molecular Cloning and Characterization of Bifidobacterium bifidum 1,2-α-l-Fucosidase (AfcA), a Novel Inverting Glycosidase (Glycoside Hydrolase Family 95)
- Analysis of the reaction coordinate of alpha-L-fucosidases: a combined structural and quantum mechanical approach.
- Comparative and phylogenetic analysis of alpha-L-fucosidase genes.
- Polysaccharide Degradation and Synthesis by Extremely Thermophilic Anaerobes
- Facile synthesis of sulfonium ion derivatives of 1,5-anhydro-5-thio-L-fucitol as potential alpha-L-fucosidase inhibitors.
- Isolation and characterization of a new family 42 beta-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residues.
- Mechanistic investigation of the endo-α-N-acetylgalactosaminidase from Streptococcus pneumoniae R6
- Biochemical and kinetic analysis of the GH3 family beta-xylosidase from Aspergillus awamori X-100.
- Directed evolution of the alpha-L-fucosidase from Thermotoga maritima into an alpha-L-transfucosidase.
- Discovery of picomolar slow tight-binding inhibitors of alpha-fucosidase.
- Functions of novel glycosidases isolated from bifidobacteria
- Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum.
- A simple enzymatic synthesis of 4-nitrophenyl beta-1,4-D-xylobioside, a chromogenic substrate for assay and differentiation of endoxylanases.
- 1,3-1,4-α-l-Fucosynthase That Specifically Introduces Lewis a/x Antigens into Type-1/2 Chains*
- Green fluorescent protein and factorial approach: an effective partnership for screening the soluble expression of recombinant proteins in Escherichia coli.
- Developing an asymmetric, stereodivergent route to selected 6-deoxy-6-fluoro-hexoses.
- Structure and Substrate Specificity of a Eukaryotic Fucosidase from Fusarium graminearum*
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