A Kinetic Analysis of the Folding of Human Carbonic Anhydrase II and Its Catalysis by Cyclophilin (*)

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Summary

The kinetics of unfolding and refolding of human carbonic anhydrase II and its catalysis by the peptidyl-prolyl-cis/trans-isomerase cyclophilin were investigated and it was found that some prolyl peptide bonds isomerize early in refolding, at the stage of the molten globule and as a consequence, molecules with incorrect prolyL isomers are formed in competition with the productive folding of Uf.

Type
article
Published
1995-01-13
Cited by
67
References
31
Access
Open access

Keywords

Chemistry, Prolyl isomerase, Isomerization, Molten globule, Peptidylprolyl isomerase

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