Effective targeted gene ‘knockdown’ in zebrafish
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Summary
It is shown here that antisense, morpholino-modified oligonucleotides (morpholinos) are effective and specific translational inhibitors in zebrafish, and conserved vertebrate processes and diseases are now amenable to a systematic, in vivo, reverse-genetic paradigm using zebra fish embryos.
- Type
- article
- Published
- 2000-10-01
- Cited by
- 2,653
- References
- 31
- OpenAlex
- https://openalex.org/W1998756807
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21451111
Keywords
Morpholino, Zebrafish, Biology, Gene knockdown, Genetics
References
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- Morpholino Antisense Oligomers: Design, Preparation, and Properties
- The EGF-CFC protein one-eyed pinhead is essential for nodal signaling.
- Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes
- The Metabolic Basis of Inherited Diseases
- The zebrafish bozozok locus encodes Dharma, a homeodomain protein essential for induction of gastrula organizer and dorsoanterior embryonic structures.
Cited by
- Molecular Mechanisms Underlying Adaptation to PAHs in Fundulus heteroclitus
- Functional analysis of human enhancers using the zebrafish embryo
- Applying transgenic zebrafish technology to study the retina.
- Vertebrate model systems in the study of early heart development: Xenopus and zebrafish.
- A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon.
- Docking protein SNT1 is a critical mediator of fibroblast growth factor signaling during Xenopus embryonic development
- Wiring the zebrafish: axon guidance and synaptogenesis.
- A novel repressor-type homeobox gene, ved, is involved in dharma/bozozok-mediated dorsal organizer formation in zebrafish.
- Double cone dystrophy and RPE degeneration in the retina of the zebrafish gnn mutant.
- A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells
- Cell-death alternative model organisms: why and which?
- Paraxial mesoderm specifies zebrafish primary motoneuron subtype identity
- Connexin 48.5 Is Required for Normal Cardiovascular Function and Lens Development in Zebrafish Embryos*[boxs]
- Harnessing zebrafish for the study of white blood cell development and its perturbation.
- Differential requirements for COPI transport during vertebrate early development.
- Requirement for proprotein convertase 5/6 during decidualization of human endometrial stromal cells in vitro.
- Culture of embryonic stem cell lines from zebrafish.
- Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity
- Pathogenesis and inflammatory response to Edwardsiella tarda infection in the zebrafish.
- The Role of Hedgehog Signaling in the Development of the Zebrafish Visual System
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