Role of tumor suppressor p53 domains in selective binding to supercoiled DNA.
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Summary
It is demonstrated here that the p53 C-terminal domain (amino acids 347-382) and a p53 oligomeric state are important for SCS binding and a model according to which two DNA duplexes are compacted into p53-scDNA filaments is proposed and a role for filament formation in recombination is discussed.
- Type
- article
- Published
- 2002-11-15
- Cited by
- 54
- References
- 59
- Access
- Open access
- OpenAlex
- https://openalex.org/W1988332041
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:14230950
Keywords
Biology, Mutant, DNA, Molecular biology, HMG-box
References
- The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted alpha helices: crystal structure of the protein-DNA complex.
- Human p53 Binds Holliday Junctions Strongly and Facilitates Their Cleavage*
- p53 is linked directly to homologous recombination processes via RAD51/RecA protein interaction.
- The dihedral symmetry of the p53 tetramerization domain mandates a conformational switch upon DNA binding.
- Binding of p53 and its core domain to supercoiled DNA.
- Eukaryotic topoisomerases recognize nucleic acid topology by preferentially interacting with DNA crossovers.
- Surfing the p53 network
- Different Regulation of the p53 Core Domain Activities 3′-to-5′ Exonuclease and Sequence-Specific DNA Binding
- Post-translational modifications and activation of p53 by genotoxic stresses.
- Analysis of p53 expression in human tumours: an antibody raised against human p53 expressed in Escherichia coli.
- Two Distinct Signaling Pathways Activate the Latent DNA Binding Function of p53 in a Casein Kinase II-independent Manner (*)
- Specific modulation of p53 binding to consensus sequence within supercoiled DNA by monoclonal antibodies.
- Visualization of supercoiled DNA with atomic force microscopy in situ.
- Role of heteroduplex joints in the functional interactions between human Rad51 and wild-type p53
- Tumor suppressor protein p53 binds preferentially to supercoiled DNA
- Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393).
- p53 blocks RuvAB promoted branch migration and modulates resolution of Holliday junctions by RuvC.
- p53 binds single-stranded DNA ends and catalyzes DNA renaturation and strand transfer.
- Intramolecular synapsis of duplex DNA by vaccinia topoisomerase
- Formation of stable p53 homotetramers and multiples of tetramers
Cited by
- Investigations of the supercoil-selective DNA binding of wild type p53 suggest a novel mechanism for controlling p53 function.
- DNA modification with cisplatin affects sequence‐specific DNA binding of p53 and p73 proteins in a target site‐dependent manner
- Cruciform structures are a common DNA feature important for regulating biological processes
- Recognition of cisplatin-damaged DNA by p53 protein: critical role of the p53 C-terminal domain.
- GFP-like fluorophores as DNA labels for studying DNA-protein interactions.
- Improving the assessment of the outcome of nonsynonymous SNVs with a consensus deleteriousness score, Condel.
- Change of the Protein p53 Electrochemical Signal According to its Structural Form – Quick and Sensitive Distinguishing of Native, Denatured, and Aggregated Form of the “Guardian of the Genome”
- Differential Salt-Induced Dissociation of the p53 Protein Complexes with Circular and Linear Plasmid DNA Substrates Suggest Involvement of a Sliding Mechanism
- The Central Region of BRCA1 Binds Preferentially to Supercoiled DNA
- Modulation of gene expression in U251 glioblastoma cells by binding of mutant p53 R273H to intronic and intergenic sequences
- Electrochemical detection of DNA binding by tumor suppressor p53 protein using osmium-labeled oligonucleotide probes and catalytic hydrogen evolution at the mercury electrode
- Preferential binding of IFI16 protein to cruciform structure and superhelical DNA.
- Sequestering of p53 into DNA-protein filaments revealed by electron microscopy.
- p53 in recombination and repair
- Superhelical DNA as a preferential binding target of 14-3-3γ protein
- Wild-type p53 stimulates homologous recombination upon sequence-specific binding to the ribosomal gene cluster repeat
- Fmoc‐based chemical synthesis and selective binding to supercoiled DNA of the p53 C‐terminal segment and its phosphorylated and acetylated derivatives
- Impact of cadmium, cobalt and nickel on sequence-specific DNA binding of p63 and p73 in vitro and in cells.
- A label-free electrochemical test for DNA-binding activities of tumor suppressor protein p53 using immunoprecipitation at magnetic beads.
- Biophysical and electrochemical studies of protein–nucleic acid interactions
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