A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.
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Summary
A sensitive silver stain for detecting bacterial lipopolysaccharides in polyacrylamide gels is developed by modifying the silver-staining method used for proteins, which is 500 times more sensitive than the periodic acid-Schiff stain and can detect less than 5 ng of rough type lipopolySaccharides.
- Type
- article
- Published
- 1982-01-01
- Cited by
- 3,108
- References
- 18
- OpenAlex
- https://openalex.org/W1985782125
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:34357775
Keywords
Stain, Silver stain, Staining, Chemistry, Polyacrylamide gel electrophoresis
References
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- Recent results on the biochemistry of the cell wall lipopolysaccharides of Salmonella bacteria.
- Biological and physicochemical properties of the lipopolysaccharide of Chromatium vinosum
- Two mutations which affect the barrier function of the Escherichia coli K-12 outer membrane
- Detection of Sugars on Paper Chromatograms
- A rapid sensitive silver stain for polypeptides in polyacrylamide gels.
- Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
- A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
- Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis.
- A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels.
- Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis.
- Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2.
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
- Ultrasensitive silver‐based color staining of polypeptides in polyacrylamide gels
- SDS-polyacrylamide gel electrophoresis of lipopolysaccharides.
- Lipid A: chemical structure and biological activity.
- Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall.
- Polyacrylamide Gel Electrophoresis of Viral Proteins
Cited by
- Improved detection of infection by Escherichia coli O157 in patients with haemolytic uraemic syndrome by means of IgA antibodies to lipopolysaccharide.
- Structural and immunochemical studies of O-specific polysaccharide of Proteus vulgaris 5/43 belonging to OX19 group (O-variants).
- Eight lipooligosaccharides of Neisseria meningitidis react with a monoclonal antibody which binds lacto-N-neotetraose (Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc)
- Isolation, purification and partial analysis of the lipopolysaccharide antigenic determinant recognized by a monoclonal antibody to Legionella pneumophila serogroup 1.
- Purification and partial characterization of outer membrane proteins P5 and P6 from Haemophilus influenzae type b
- Conservation of epitopes in the oligosaccharide portion of the lipooligosaccharide of Haemophilus influenzae type b
- O-polysaccharide-protein conjugates induce high levels of specific antibodies to Pseudomonas aeruginosa immunotype 3 lipopolysaccharide.
- Structural analysis of lipopolysaccharides from Eikenella corrodens by use of murine monoclonal antibodies
- The core region of Proteus mirabilis R110/1959 lipopolysaccharide.
- Lipopolysaccharide mutants of Rhizobium meliloti are not defective in symbiosis
- Influence of growth temperature and lipopolysaccharide on hemolytic activity of Serratia marcescens
- Lipopolysaccharide alteration is associated with induced resistance of Neisseria gonorrhoeae to killing by human serum.
- Hafnia alvei lipopolysaccharides: isolation, sugar composition and SDS-PAGE analysis.
- Oral vaccination. Identification of classes of proteins that provoke an immune response upon oral feeding
- Lipopolysaccharides of Thiocystis violacea, Thiocapsa pfennigii, and Chromatium tepidum, species of the family Chromatiaceae
- New cysE-pyrE-linked rfa mutation in Escherichia coli K-12 that results in a heptoseless lipopolysaccharide
- Lipopolysaccharide subtypes of Haemophilus influenzae type b from an outbreak of invasive disease
- Immunologically related ketodeoxyoctonate-containing structures in Chlamydia trachomatis, Re mutants of Salmonella species, and Acinetobacter calcoaceticus var. anitratus
- The purification and protective capacity of Bordetella pertussis outer membrane proteins.
- Structure of the O-specific polysaccharide of the O23 antigen (LPS) from Escherichia coli O23:K?:H16.
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