m5C RNA and m5C DNA methyl transferases use different cysteine residues as catalysts.
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Summary
Surprisingly, the mutant of the putative Cys catalyst in the ProCys sequence was active and formed a covalent complex with 5-fluorocytosine-containing RNA, whereas the mutant at the other conserved Cys was inactive and unable to form the complex.
- Type
- article
- Published
- 2000-07-18
- Cited by
- 113
- References
- 18
- Access
- Open access
- OpenAlex
- https://openalex.org/W1984451904
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:40980851
Keywords
RNA, DNA, Conserved sequence, Biology, Transfer RNA
References
- Mutagenic Analysis of Double-stranded RNA Adenosine Deaminase, a Candidate Enzyme for RNA Editing of Glutamate-gated Ion Channel Transcripts (*)
- Specific labeling of 3' termini of RNA with T4 RNA ligase.
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- Single-step purification of T7 RNA polymerase with a 6-histidine tag.
- Identification of the catalytic nucleophile of tRNA (m5U54)methyltransferase.
- In vitro methylation of Escherichia coli 16S rRNA by tRNA (m5U54)-methyltransferase.
- DNA modification by methyltransferases.
- Identification of the 16S rRNA m5C967 methyltransferase from Escherichia coli.
- Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes.
- Covalent adducts between tRNA (m5U54)-methyltransferase and RNA substrates.
- Thiation of Nucleosides. IV. The Synthesis of 5-Fluoro-2'-deoxycytidine and Related Compounds1,2
- Evolutionary origins of apoB mRNA editing: catalysis by a cytidine deaminase that has acquired a novel RNA-binding motif at its active site.
- Structure and function of DNA methyltransferases.
- Exposition of a family of RNA m(5)C methyltransferases from searching genomic and proteomic sequences.
- Nop2p is required for pre-rRNA processing and 60S ribosome subunit synthesis in yeast
- A conserved motif in the yeast nucleolar protein Nop2p contains an essential cysteine residue.
- Expanding the Potential of DNA for Binding and Catalysis: Highly Functionalized dUTP Derivatives That Are Substrates for Thermostable DNA Polymerases.
- The catalytic mechanism and structure of thymidylate synthase.
Cited by
- Structural and evolutionary bioinformatics of the SPOUT superfamily of methyltransferases
- RNA 5-Methylcytosine Analysis by Bisulfite Sequencing.
- 1 Protein Methyltransferases: Their Distribution Among the Five Structural Classes of AdoMet-Dependent Methyltransferases.
- Etudes structurales de différents processus biologiques impliquant les ARN de transfert
- Biochemical characterisation of tRNA-Asp methyltransferase Dnmt2 and its physiological significance
- Natural history of eukaryotic DNA methylation systems.
- Conservation of tRNA and rRNA 5-methylcytosine in the kingdom Plantae
- The structure of the RNA m5C methyltransferase YebU from Escherichia coli reveals a C-terminal RNA-recruiting PUA domain.
- Histone deacetylase and DNA methyltransferase in human prostate cancer.
- AdoMet-dependent methylation, DNA methyltransferases and base flipping.
- Crystal structure of human p120 homologue protein PH1374 from Pyrococcus horikoshii
- Repair of DNA covalently linked to protein.
- THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domain
- NSun2-Mediated Cytosine-5 Methylation of Vault Noncoding RNA Determines Its Processing into Regulatory Small RNAs
- Mechanism-based strategies for trapping and crystallizing complexes of RNA-modifying enzymes.
- Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively
- Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase.
- Sequence-structure-function studies of tRNA:m5C methyltransferase Trm4p and its relationship to DNA:m5C and RNA:m5U methyltransferases.
- Crystal structure of Methanocaldococcus jannaschii Trm4 complexed with sinefungin.
- YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962.
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