"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
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Summary
The detection of murine leukemia virus antigens in complex cellular lysates was used to demonstrate the efficacy of this electrophoretic transfer technique.
- Type
- article
- Published
- 1981-04-01
- Cited by
- 7,884
- References
- 26
- OpenAlex
- https://openalex.org/W2111817213
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:39386327
Keywords
Nitrocellulose, Sodium dodecyl sulfate, Blot, Chemistry, Polyacrylamide
References
- Second conference on macromolecular synthesis: biosynthesis, modification and processing of cellular and viral polyproteins.
- Handbook of experimental immunology
- gag-Related polyproteins of Moloney murine leukemia virus: evidence for independent synthesis of glycosylated and unglycosylated forms
- Interstrain Variation of the Major Internal Structural Component (p30gag) of Two Murine Oncornaviruses: Comparative Immunochemical, Biochemical, and Biophysical Analysis
- Translational products of type-C RNA tumor viruses.
- Bacteriophage T4 prehead proteinase. II. Its cleavage from the product of gene 21 and regulation in phage-infected cells.
- High resolution two-dimensional electrophoresis of proteins.
- Studies of the Mechanism of Enhancement of Human Adenovirus Infection in Monkey Cells by Simian Virus 40
- Identification of the Gross cell surface antigen associated with murine leukemia virus-infected cells
- Structural Proteins of Mammalian Oncogenic RNA Viruses: Multiple Antigenic Determinants of the Major Internal Protein and Envelope Glycoprotein
- A core polyprotein of murine leukemia virus on the surface of mouse leukemia cells.
- X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I.
- Effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
- An efficient mRNA-dependent translation system from reticulocyte lysates.
- Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets.
- Sodium dodecylsulfate-dependent anomalies in gel electrophoresis: alterations in the banding patterns of foot-and-mouth disease virus polypeptides.
- The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses.
- The detection of DNA-binding proteins by protein blotting
- Physical and chemical properties of Moloney murine leukemia virus p30 protein: a major core structural component exhibiting high helicity and self-association.
- Sodium dodecyl sulfate in protein chemistry.
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- A major immunogenic 36,000-molecular-weight antigen from Mycobacterium leprae contains an immunoreactive region of proline-rich repeats
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- Quantification of glial fibrillary acidic protein: comparison of slot-immunobinding assays with a novel sandwich ELISA.
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