Simple and rapid fluorimetric method for DNA microassay.
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Summary
4′,6-Diamidino-2-phenylindole · 2HCl (DAPI) forms a specific complex with DNA, and this fact has been used for the quantitative fluorimetric estimation of DNA.
- Type
- article
- Published
- 1977-11-01
- Cited by
- 474
- References
- 9
- OpenAlex
- https://openalex.org/W2013672165
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:46591672
Keywords
DAPI, DNA, Chemistry, Fluorescence, Bivalent (engine)
References
- The use of anti-tumour antibiotics for simple quantitative assays for DNA.
- A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses
- Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum.
- A simple, rapid microassay for DNA
- Kinetics of renaturation of DNA.
- Use of a sequence-specific DNA-binding ligand to probe the environments of EcoRI restriction endonuclease cleavage sites.
- DAPI fluorescence of plant chromosomes prestained with actinomycin D.
- A new fluorometric method for RNA and DNA determination.
- Trypanocide Diamidine des 2‐Phenyl‐benzofurans, 2‐Phenyl‐indens und 2‐Phenyl‐indols
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- Application of a novel fluorescence probe in the determination of nucleic acids.
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