Immunochemical detection of the alpha-subunit of the G-protein, GZ, in membranes and cytosols of mammalian cells.
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Summary
An antiserum was raised against a synthetic peptide deduced from published cDNA sequences of the alpha-subunit of the putative G-protein, GZ, and failed to detect thealpha-sub unit of GZ in brain membranes.
- Type
- article
- Published
- 1988-12-30
- Cited by
- 31
- References
- 16
- OpenAlex
- https://openalex.org/W1977242067
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:46469635
Keywords
Cytosol, Protein subunit, Antiserum, Membrane, Molecular biology
References
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- The regulatory component of adenylate cyclase. Purification and properties.
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- Chromatographic resolution and immunologic identification of the alpha 40 and alpha 41 subunits of guanine nucleotide-binding regulatory proteins from bovine brain.
- G protein involvement in receptor-effector coupling.
- Identification of a new GTP-binding protein. A Mr = 43,000 substrate for pertussis toxin.
- Protein measurement with the Folin phenol reagent.
- A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.
- Evidence for involvement of guanine nucleotide‐binding regulatory proteins in the activation of phospholipases by hormones
- Identification and characterization of the 35-kDa beta subunit of guanine-nucleotide-binding proteins by an antiserum raised against transducin.
- Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go
- Subcellular localization and quantitation of the major neutrophil pertussis toxin substrate, Gn
- G protein multiplicity in eukaryotic signal transduction systems.
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Cited by
- Identification of Gz alpha as a pertussis toxin-insensitive G protein in human platelets and megakaryocytes.
- Platelets and Megakaryocytes
- Biology of Cellular Transducing Signals
- Novel localization of a G protein, Gz-alpha, in neurons of brain and retina
- G-proteins and second messengers in mitogenesis.
- Identification of multiple Gi subtypes and a novel G protein in bovine kidney cortex.
- Intracellular mechanisms for alpha 1‐adrenergic regulation of the transient outward current in rabbit atrial myocytes.
- Enzymatic and immunological detection of a G‐protein in Halobacterium halobium
- Regulation of guanine nucleotide binding regulatory proteins in cultured adrenal cells by adrenocorticotropin and angiotensin-II.
- Identification of heterotrimeric G proteins in human sperm tail membranes
- Gzα deficient mice: enzyme levels in the autonomic nervous system, neuronal survival and effect of genetic background
- Localization of G-protein in rat occipital cerebral cortex.
- Signal transduction through G proteins in the cardiac myocyte.
- Endothelin/sarafotoxin receptor induced phosphoinositide turnover: effects of pertussis and cholera toxins and of phorbol ester.
- Phosphorylation of G-protein alpha-subunits in intact adipose cells: evidence against a mediating role in insulin-dependent metabolic effects.
- Localization of several G-protein subunits in human kidney.
- The G-protein family and their interaction with receptors.
- Inhibition of calcium currents in cultured myenteric neurons by neuropeptide Y: evidence for direct receptor/channel coupling.
- Localization and polar distribution of several G-protein subunits along nephron segments.
- Growth factor second messenger systems: Oncogenes and the heterotrimeric GTP‐;binding protein connection
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