“Self-tanning”—a new and important source of stoichiometric error in cytophotometric determination of nuclear DNA content in plants
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Summary
The rationale for using formaldehyde as a fixative in Feulgen-cytophotometry can be mainly seen in its capability of eliminating the “self-tanning error”.
- Type
- article
- Published
- 2004-01-01
- Cited by
- 99
- References
- 16
- OpenAlex
- https://openalex.org/W1976454260
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:9506464
Keywords
Feulgen stain, Fixation (population genetics), Biology, Nuclear DNA, Formaldehyde
References
- Angewandte Statistik : statistische Methoden und ihre Anwendungen
- SOME CHARACTERISTICS OF THE COLD HYDROLYSIS TECHNIQUE FOR STAINING PLANT TISSUES BY THE FEULGEN REACTION
- H. KRUG: Histo- und Zytophotometrie. 146 Seiten, 36 Abb. und 5 Tab. VEB Gustav Fischer Verlag, Jena 1980. Preis: 31,- M.
- DNA content polymorphism and tissue culture regeneration in Caribbean pine
- REQUIREMENT OF AN INTERNAL STANDARD FOR MICROSPECTROPHOTOMETRIC MEASUREMENTS OF DNA
- Severely distorted Feulgen-DNA amounts in Pinus (Coniferophytina) after nonadditive fixations as a result of meristematic self-tanning with vacuole contents
- Nuclear DNA and heterochromatin contents in theScilla hohenackeri group,S. persica, andPuschkinia scilloides (Liliaceae)
- Genetic instability of multiple buds of Pinuscoulteri regenerated from tissue culture
- Characterization of heterochromatin in different species of the Scilla siberica group (Liliaceae) by in situ hybridization of satellite DNAs and fluorochrome banding
- A comparison of internal standards for plant cytophotometry.
- Genome size in gymnosperms
- Nonspecific light loss and intrinsic DNA variation problems associated with feulgen DNA cytophotometry.
- Nuclear dna amounts in angiosperms.
- Mikroskopisch-chemischer Nachweis einer Nucleinsäure vom Typus der Thymonucleinsäure und die- darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten.
- Geographical Variation of Genome Size at Low Taxonomic Levels in the Scilla bifolia Alliance (Hyacinthaceae))
- Kinetics of polymerization of (+)-catechin with formaldehyde
- Angewandte Statistik
Cited by
- Investigation on the causes of stoichiometric error in genome size estimation using heat experiments: consequences on data interpretation.
- Real-time polymerase chain reaction (PCR) quantitative detection of Brassica napus using a locked nucleic acid TaqMan probe.
- Intraspecific phenotypic variation associated with nuclear DNA content in Lolium perenne L.
- A comparative investigation of nuclear DNA content and its phenotypic impacts in Silene marizii and S. latifolia
- Nuclear DNA Content Analysis of Plant Seeds by Flow Cytometry
- DNA content variation in monilophytes and lycophytes: large genomes that are not endopolyploid
- Chromosome banding and genome size differentiation inProspero (Hyacinthaceae): Diploids
- Chromosome numbers in some Artemisia (Asteraceae, Anthemideae) species and genome size variation in its subgenus Dracunculus: Karyological, systematic and phylogenetic implications
- Cytochemistry and C-values: the less-well-known world of nuclear DNA amounts.
- Are seeds suitable for flow cytometric estimation of plant genome size?
- Chromosome numbers, karyotypes and nuclear DNA contents from perennial polyploid groups ofCerastium (Caryophyllaceae)
- Does genome size in Dasypyrum villosum vary with fruit colour?
- Smallest angiosperm genomes found in lentibulariaceae, with chromosomes of bacterial size.
- Plant DNA flow cytometry and estimation of nuclear genome size.
- Nuclear size, DNA content, and chromatin condensation are different in individual tissues of the maize root apex
- High resolution bands in maize chromosomes by G-banding methods
- A Survey of Karyological Phenomena in the Juncaceae with Emphasis on Chromosome Number Variation and Evolution
- Nuclear DNA variability withinPisum sativum (Leguminosae): Cytophotometric analyses
- Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry—new prospects for plant research
- Genome size of Adriatic seagrasses
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