Real-time polymerase chain reaction (PCR) quantitative detection of Brassica napus using a locked nucleic acid TaqMan probe.
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Summary
The Brassica napus-specific PCR assay was demonstrated to be effective for the detection of processed food samples and for the quantification of Roundup Ready canola content in mixed samples.
- Type
- article
- Published
- 2006-01-21
- Cited by
- 8
- References
- 28
- Access
- Open access
- OpenAlex
- https://openalex.org/W16478231
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21181115
Keywords
Fifteenth, History, Art, Ancient history
References
- Novel reference gene, High-mobility-group protein I/Y, used in qualitative and real-time quantitative polymerase chain reaction detection of transgenic rapeseed cultivars.
- Roundup Ready® soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses
- “Self-tanning”—a new and important source of stoichiometric error in cytophotometric determination of nuclear DNA content in plants
- Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods.
- A rapeseed-specific gene, acetyl-CoA carboxylase, can be used as a reference for qualitative and real-time quantitative PCR detection of transgenes from mixed food samples.
- Real-time genotyping with oligonucleotide probes containing locked nucleic acids.
- Use of locked nucleic acid oligonucleotides as hybridization/FRET probes for quantification of 16S rDNA by real-time PCR.
- Validation of a cotton-specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons
- Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA.
- Detection and quantification of roundup ready soy in foods by conventional and real-time polymerase chain reaction.
- LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition
- Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products
- Validation of real-time PCR methods for the quantification of transgenic contaminations in rape seed
- Risks of gene flow from transgenic herbicide-resistant canola (Brassica napus) to weedy relatives in southern Australian cropping systems
- Efficient priming of PCR with short oligonucleotides conjugated to a minor groove binder
- Enhanced allele‐specific PCR discrimination in SNP genotyping using 3′ locked nucleic acid (LNA) primers
- The conformations of locked nucleic acids (LNA)
- Evaluation of the performance of LNA and MGB probes in 5'-nuclease PCR assays.
- Genotyping of the triallelic variant G2677T/A in MDR1 using LightCycler with locked-nucleic-acid-modified hybridization probes.
- Event-specific plasmid standards and real-time PCR methods for transgenic Bt11, Bt176, and GA21 maize and transgenic GT73 canola.
Cited by
- Species-specific identification of seven vegetable oils based on suspension bead array.
- Extraction of RNA from fresh, frozen, and lyophilized tuber and root tissues.
- Instantaneous derivatization technology for the simultaneous and homogeneous detection of multiple double-stranded PCR amplicons.
- A strategy for designing multi-taxa specific reference gene systems. example of application--ppi phosphofructokinase (ppi-PPF) used for the detection and quantification of three taxa: maize (Zea mays), cotton (Gossypium hirsutum) and rice (Oryza sativa).
- Advances in molecular techniques for the detection and quantification of genetically modified organisms
- Detection of reverse transcription-PCR products by a simple and rapid light scattering technique.
- Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms
- Fluorescent Oligonucleotide Probes for the Quantification of RNA by Real-Time qPCR.
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