Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca
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Summary
A plasmid containing the NPT II structural gene (neomycin phosphotransferase) fused to the N-terminal region of a homologous actin gene was used for the transformation of Absidia glauca protoplasts, providing evidence that pAmN61 is autonomously replicated in Absidiaglauca.
- Type
- article
- Published
- 2004-01-01
- Cited by
- 39
- References
- 14
- Access
- Open access
- OpenAlex
- https://openalex.org/W1974268167
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:23925938
Keywords
Biology, Selectable marker, Plasmid, Transformation (genetics), Southern blot
References
- Protein fusions with the kanamycin resistance gene from transposon Tn5.
- In vitro packaging of lambda and cosmid DNA.
- Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid.
- Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
- Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
- Screening lambdagt recombinant clones by hybridization to single plaques in situ.
- Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
- Strain-dependent variation in ribosomal DNA arrangement in Absidia glauca.
- A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13.
- Autonomous replication of plasmids in Mucor transformants
- Transformation of yeast.
- Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
- A complementation analysis of the restriction and modification of DNA in Escherichia coli.
- Detection of specific sequences among DNA fragments separated by gel electrophoresis.
Cited by
- High efficiency transformation of Penicillium nalgiovense with integrative and autonomously replicating plasmids.
- Molecular characterization of the horizontal gene transfer between mycoparasite, parasitella parasitica, and its host, absidia glauca
- Genetic damage following introduction of DNA in Phycomyces.
- Heterologous transformation of Mucor circinelloides with the Phycomyces blakesleeanus leul gene
- Molecular cloning and sequence analysis of the scpZ gene encoding the serine carboxypeptidase of Absidia zychae
- Characterisation of the Mucor circinelloides regulated promoter gpd1P
- Transfer of genetic information from the mycoparasite Parasitella parasitica to its host Absidia glauca
- Plasmids for expression of heterologous proteins in Rhizopus oryzae
- Circular extrachromosomal DNA codes for a surface protein in the (+) mating type of the zygomycete Absidia glauca
- Transformation of the mycoparasite Parasitella simplex to neomycin resistance
- Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides
- Integrative transformation of a zygomycete, Absidia glauca, with vectors containing repetitive DNA
- Homologous recombination and double-strand break repair in the transformation of Rhizopus oryzae
- Transformation of the fungus Absidia glauca by complementation of a methionine-auxotrophic strain affected in the homoserine-acetyltransferase gene
- A nucleotide sequence involved in replicative transformation of a filamentous fungus
- Isolation, characterization and transformation, by autonomous replication, ofMucor circinelloides OMPdecase-deficient mutants
- Gene replacement and ectopic integration in the zygomycete Mucor circinelloides
- A multicopy vector system for genetic studies in Mucor circinelloides and other zygomycetes
- Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene
- Microprojectile bombardment as a reliable method for transformation of the mucoralean fungus Absidia glauca
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