Measurements of the free luminal ER Ca(2+) concentration with targeted "cameleon" fluorescent proteins.
Explore this paper's citation graph
Summary
The advantages and pitfalls of using the genetically encoded "cameleon" Ca(2+) indicators for ER Ca( 2+) measurements are discussed.
- Type
- review
- Published
- 2003-08-01
- Cited by
- 122
- References
- 52
- Access
- Open access
- OpenAlex
- https://openalex.org/W1973828083
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10405126
Keywords
Aequorin, Förster resonance energy transfer, Green fluorescent protein, Fluorescence, Biophysics
References
- Rapid Turnover of Calcium in the Endoplasmic Reticulum during Signaling
- Monitoring dynamic changes in free Ca2+ concentration in the endoplasmic reticulum of intact cells.
- Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
- FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule
- Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein.
- Overexpression of Calreticulin Increases Intracellular Ca Storage and Decreases Store-operated Ca Influx (*)
- Differential Regulation of Active Zone Density during Long-Term Strengthening of Drosophila Neuromuscular Junctions
- Detection in Living Cells of Ca2+-dependent Changes in the Fluorescence Emission of an Indicator Composed of Two Green Fluorescent Protein Variants Linked by a Calmodulin-binding Sequence
- A Molecular Sensor Detects Signal Transduction from Caveolae in Living Cells*
- Noninvasive measurement of the pH of the endoplasmic reticulum at rest and during calcium release.
- Reducing the Environmental Sensitivity of Yellow Fluorescent Protein
- Visualization of biphasic Ca2+ diffusion from cytosol to nucleus in contracting adult rat cardiac myocytes with an ultra-fast confocal imaging system.
- Dynamic and quantitative Ca2+ measurements using improved cameleons.
- Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons.
- The Ca2+ concentration of the endoplasmic reticulum is a key determinant of ceramide‐induced apoptosis: significance for the molecular mechanism of Bcl‐2 action
- Technique for in situ measurement of calcium in intracellular inositol 1,4,5-trisphosphate-sensitive stores using the fluorescent indicator mag-fura-2.
- Changes in Endoplasmic Reticulum Luminal Environment Affect Cell Sensitivity to Apoptosis
- Circularly permuted green fluorescent proteins engineered to sense Ca2+
- Heterogeneity of channel density in inositol-1,4,5-trisphosphate-sensitive Ca2+ stores
- Calcium homeostasis in identified rat gonadotrophs.
Cited by
- The molecular choreography of a store-operated calcium channel
- Studies on the resolution of subcellular free calcium concentrations: a technological advance. Focus on "detection of differentially regulated subsarcolemmal calcium signals activated by vasoactive agonists in rat pulmonary artery smooth muscle cells".
- Systematic analysis of Ca2+ homeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles
- Handbook of ion channels
- Store-operated Ca(2+) entry.
- How to make a good egg!: The need for remodeling of oocyte Ca(2+) signaling to mediate the egg-to-embryo transition.
- Fluorescent measurement of [Ca2+]c: basic practical considerations.
- Role of the Cytoplasmic Polyadenylation Element Binding Proteins in Neuron: A Dissertation
- Calmodulin as a regulator of circadian clock function and photoperiodic flowering in Arabidopsis thaliana
- Role of STIM (Stromal interaction molecule) in Store operated calcium entry
- Genetically-encoded probes for measurement of intracellular calcium
- Analysis of CPEB Family Protein Member CPEB4 Function in Mammalian Neurons: A Dissertation
- A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+
- Store-Operated Orai Channels: Structure and Function
- Taxol Directly Induces Endoplasmic Reticulum‐Associated Calcium Changes That Promote Apoptosis in Breast Cancer Cells
- Nouveaux mécanismes de régulation de la concentration calcique réticulaire : implication dans la physiopathologie de la prostate humaine.
- The Endoplasmic Reticulum: Folding, Calcium Homeostasis, Signaling, and Redox Control
- Morphological and functional aspects of STIM1-dependent assembly and disassembly of store-operated calcium entry complexes.
- Photoproteins: Important New Tools in Drug Discovery
- Protein Conformational Switches: From Nature to Design
Related papers
- Fusion of Aequorea victoria GFP and aequorin provides their Ca(2+)-induced interaction that results in red shift of GFP absorption and efficient bioluminescence energy transfer.
- Isolation and characterization of a photoprotein, “phialidin”, and a spectrally unique green-fluorescent protein from the bioluminescent jellyfish Phialidium gregarium
- Measurements of Ca²⁺ concentration with recombinant targeted luminescent probes.
- Photoproteins as luminescent labels in binding assays
- Green fluorescent protein in retroviral vector constructs as marker and reporter of gene expression for cell and gene therapy applications.
- Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level.
- Fluorescent Protein–photoprotein Fusions and Their Applications in Calcium Imaging
- Using targeted variants of aequorin to measure Ca2+ levels in intracellular organelles.
- GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles