FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule
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Summary
A new cameleon is rationally designed that displays a two-fold increase in the FRET dynamic range within the physiologically significant range of cytoplasmic Ca2+ concentration of 0.05-1 μM.
- Type
- article
- Published
- 2001-12-01
- Cited by
- 217
- References
- 32
- OpenAlex
- https://openalex.org/W1557879301
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:391648
Keywords
Förster resonance energy transfer, Calmodulin, Green fluorescent protein, Organelle, Biophysics
References
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- The versatility and universality of calcium signalling
- The histamine H1 receptor in GT1‐7 neuronal cells is regulated by calcium influx and KN‐62, a putative inhibitor of calcium/calmodulin protein kinase II
- Intracellular Ca2+ measurement with Indo-1 in substrate-attached cells: advantages and special considerations.
- A calmodulin-target peptide hybrid molecule with unique calcium-binding properties.
- Measurement of cytosolic free Ca2+ in individual small cells using fluorescence microscopy with dual excitation wavelengths.
- Improved green fluorescence
- Target enzyme recognition by calmodulin: 2.4 A structure of a calmodulin-peptide complex.
- The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo.
- Dynamic and quantitative Ca2+ measurements using improved cameleons.
- Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.
- Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
- Circularly permuted green fluorescent proteins engineered to sense Ca2+
- Solution structure of a calmodulin-target peptide complex by multidimensional NMR.
- Modulation of calmodulin plasticity in molecular recognition on the basis of x-ray structures.
- Wavelength mutations and posttranslational autoxidation of green fluorescent protein.
Cited by
- Techniques to dissect cellular and subcellular function in the Drosophila nervous system.
- Calcium indicators based on calmodulin-fluorescent protein fusions.
- Current approaches for engineering proteins with diverse biological properties.
- FRET evidence for a conformational change in TFIIB upon TBP-DNA binding.
- EVALUATION OF AMPEROMETRIC SENSORS CONTAINING ENZYMES IMMOBILIZED IN HYDROGELS THAT INCORPORATE THE REDOX MEDIATOR
- Fluorescent measurement of [Ca2+]c: basic practical considerations.
- Local Protein Turnover As a Regulatory Mechanism of Growth and Collapse of Neuronal Growth Cones
- Design and Synthesis of Boronic Acid-Based Sensors for Microarray Analysis and FRET-detection to study Carbohydrates
- Molecular Biophysics for the Life Sciences
- Redesign of calcium-regulated protein aequorin towards the development of a novel ion bioreporter
- Development and application of multiplexed fluorescence imaging to chemotaxis signalling pathways
- The Theodore Bücher lecture. Investigating signal transduction with genetically encoded fluorescent probes.
- Analysis of integrin signaling by fluorescence resonance energy transfer.
- Development and analysis of recombinant fluorescent probes for use in live cell imaging of filamentous fungi
- Genetically-encoded probes for measurement of intracellular calcium
- Genetically encoded Ca2+ indicators: using genetics and molecular design to understand complex physiology
- Molecular spies for bioimaging--fluorescent protein-based probes.
- Looking forward to seeing calcium
- Dynamic visualization of cellular signaling.
- Graphene‐Oxide‐Conjugated Polymer Hybrid Materials for Calmodulin Sensing by Using FRET Strategy
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