Rapid analysis of amino acids using pre-column derivatization.
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Summary
The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydrolyzates and the liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids.
- Type
- article
- Published
- 1984-12-07
- Cited by
- 2,125
- References
- 10
- OpenAlex
- https://openalex.org/W1967647423
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:36194496
Keywords
Derivatization, Chromatography, Amino acid, Chemistry, Derivative (finance)
References
- Condensation of ninhydrin with aldehydes and primary amines to yield highly fluorescent ternary products. I. Studies on the mechanism of the reaction and some characteristics of the condensation product.
- Chromatography of amino acids on sulfonated polystyrene resins.
- Separation of amino acids by high performance liquid chromatography.
- Ion Exchange Chromatography of Amino Acids. A Single Column, High Resolving, Fully Automatic Procedure.
- High performance liquid chromatographic determination of amino acids in the picomole range.
- Novel reagent for the fluorometric assay of primary amines
- Separations on heavily loaded small particle columns in high speed liquid chromatography
- Rapid measurement of free amino acids in serum and CSF using high-performance liquid chromatography.
- Fluorescence reaction for amino acids.
- “Reversed phase”-Hochdruck-Flüssigkeits-Chromatographie von PTH-Aminosäuren
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- The effect of substituting phosphotyrosine for sulphotyrosine on the activity of hirudin.
- Cystic fibrosis transmembrane conductance regulator: nucleotide binding to a synthetic peptide
- A key amino acid determining G3m(b) allotypic markers
- Effect of tertiary amine on the carbodiimide-mediated peptide synthesis.
- Carassin: A Tachykinin That Is Structurally Related to Neuropeptide‐γ from the Brain of the Goldfish
- Amino acid sequence of growth hormone isolated from medium of incubated pituitary glands of tilapia (Oreochromis mossambicus).
- Monoclonal antibodies to denatured ragweed pollen allergen Amb a I: characterization, specificity for the denatured allergen, and utilization for the isolation of immunogenic peptides of Amb a I.
- Soluble lactose-binding vertebrate lectins: a growing family.
- Cleavage of a model peptide at its glycine residue by alkaline mercuric oxycyanide.
- Hexokinase receptor complex in hepatoma mitochondria: evidence from N,N'-dicyclohexylcarbodiimide-labeling studies for the involvement of the pore-forming protein VDAC.
- Profiling of amino acids in body fluids and tissues by means of liquid chromatography.
- Characterization of an amidated form of pancreatic polypeptide from the daddy sculpin (Cottus scorpius).
- Isolation and characterization of ornitho-kininogen.
- Amino acid sequence similarity between malate dehydrogenases (NAD) and pea chloroplast malate dehydrogenase (NADP).
- Amino acid sequence of rubredoxin from Desulfovibrio desulfuricans strain 27774
- Isolation and biological activity of [Trp5]bradykinin from the plasma of the phylogenetically ancient fish, the bowfin and the longnosed gar.
- Pulsed EPR studies of the binuclear Mn(III)Mn(IV) center in catalase from Thermus thermophilus.
- Probing the structure of human growth hormone by limited proteolysis.
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