The effect of substituting phosphotyrosine for sulphotyrosine on the activity of hirudin.
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Summary
It was found that the introduction of the negatively charged phosphate had fully restored the affinity of recombinant hirudin for alpha-thrombin to the level of the wild-type sulphatohirud in.
- Type
- article
- Published
- 1990-02-01
- Cited by
- 55
- References
- 26
- OpenAlex
- https://openalex.org/W1690647
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:30166137
Keywords
Computer science
References
- Identification of regions of alpha-thrombin involved in its interaction with hirudin.
- Determination of the operational molarity of solutions of bovine alpha-chymotrypsin, trypsin, thrombin and factor Xa by spectrofluorimetric titration.
- Amino acid sequence of the phosphorylation site of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
- Identification of two sites of sulfation of human heparin cofactor II.
- The use of sequence-specific antibodies to identify a secondary binding site in thrombin.
- The hirudin-binding site of human alpha-thrombin. Identification of lysyl residues which participate in the combining site of hirudin-thrombin complex.
- Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi.
- Detection and quantification of phosphotyrosine in proteins.
- Rapid analysis of amino acids using pre-column derivatization.
- Protein tyrosine sulfation
- Use of site-directed mutagenesis to investigate the basis for the specificity of hirudin.
- Primary structures of new ‘iso‐hirudins’
- Quantitative evaluation of the contribution of ionic interactions to the formation of the thrombin-hirudin complex.
- Kinetics of the inhibition of thrombin by hirudin.
- Protein-Tryosine Kinases
- Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis.
- Contribution of the N-terminal region of hirudin to its interaction with thrombin.
- Expression and Secretion in S. Cerevisiae of Biologically Active Leech Hirudin
- Quantitation of aspartate and glutamate in HPLC analysis of phenylthiocarbamyl amino acids.
- Sulphation of tyrosine residues—a widespread modification of proteins
Cited by
- New anticoagulant drugs.
- Tyrosine O‐sulfation promotes proteolytic processing of progastrin.
- Regulation of thrombin activity--pharmacologic and structural aspects.
- Solid-phase synthesis of a range of O-phosphorylated peptides by post-assembly phosphitylation and oxidation.
- Reevaluating the effects of tyrosine iodination of recombinant hirudin on its thrombin inhibition kinetics.
- Vacuolar chitinases of tobacco: a new class of hydroxyproline-containing proteins.
- Residues 287-301 of human ribonuclease inhibitor do not affect ribonuclease activity and inhibitor binding; a reply.
- Sulphation of hirudin in BHK cells
- NMR solution structure of a novel hirudin variant HM2, N-terminal 1-47 and N64-->V + G mutant.
- Chemical activation of MEK1--a redox trigger for evaluating the effects of phosphorylation.
- Enzymic O-sulfation of tyrosine residues in hirudins by sulfotransferase from Eubacterium A-44.
- Protein tyrosine sulfation, 1993--an update.
- Recombinant Hirudin: Genetic Engineering and Structure Analysis
- Role of interactions involving C-terminal nonpolar residues of hirudin in the formation of the thrombin-hirudin complex.
- Lepirudin
- Gerinnungshemmende Wirkstoffe blutsaugender Tiere: von Hirudin zu Hirudinmimetica
- Different specificities of spleen tyrosine protein kinases for synthetic peptide substrates.
- Substitution of phosphotyrosine for sulphotyrosine in biologically active peptides. Enzymatic phosphorylation of a progastrin peptide confers immunoreactivity reminiscent of the sulphated derivative.
- Strategies for the design of novel thrombolytic and antithrombolytic agents.
- Current status of the anticoagulant hirudin: its biotechnological production and clinical practice
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