Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand
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Summary
A facile approach for designing families of GPCRs with engineered ligand specificities will prove to be powerful tools for selectively modulating signal-transduction pathways in vitro and in vivo.
- Type
- article
- Published
- 2007-03-20
- Cited by
- 1,979
- References
- 36
- Access
- Open access
- OpenAlex
- https://openalex.org/W1966403223
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:26704698
Keywords
G protein-coupled receptor, Cell biology, Receptor, Muscarinic acetylcholine receptor, Biology
References
- Engineering receptors activated solely by synthetic ligands (RASSLs).
- Functional role of proline and tryptophan residues highly conserved among G protein‐coupled receptors studied by mutational analysis of the m3 muscarinic receptor.
- Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method.
- Multiple Muscarinic Acetylcholine Receptor Subtypes Modulate Striatal Dopamine Release, as Studied with M1–M5 Muscarinic Receptor Knock-Out Mice
- Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice
- Allele-specific activation of genetically engineered receptors.
- Role of conserved threonine and tyrosine residues in acetylcholine binding and muscarinic receptor activation. A study with m3 muscarinic receptor point mutants.
- Functional expression of M1, M3 and M5 muscarinic acetylcholine receptors in yeast
- Controlling signaling with a specifically designed Gi-coupled receptor.
- A simple and highly efficient procedure for rescuing autonomous plasmids from yeast.
- Fast noninvasive activation and inhibition of neural and network activity by vertebrate rhodopsin and green algae channelrhodopsin.
- N-desmethylclozapine, an allosteric agonist at muscarinic 1 receptor, potentiates N-methyl-d-aspartate receptor activity
- The receptors for mammalian sweet and umami taste.
- Hyperactivity, elevated dopaminergic transmission, and response to amphetamine in M1 muscarinic acetylcholine receptor-deficient mice
- Reversible metabolism of clozapine and clozapine N-oxide in schizophrenic patients.
- The role of M1 muscarinic receptor agonism of N-desmethylclozapine in the unique clinical effects of clozapine
- A Single Mutation in the 5-HT4 Receptor (5-HT4-R D100(3.32)A) Generates a Gs-coupled Receptor Activated Exclusively by Synthetic Ligands (RASSL)*
- Identification of an Agonist-induced Conformational Change Occurring Adjacent to the Ligand-binding Pocket of the M3 Muscarinic Acetylcholine Receptor*
- Mutagenesis reveals a role for ABP/GRIP binding to GluR2 in synaptic surface accumulation of the AMPA receptor.
- Sindbis vector SINrep(nsP2S726): a tool for rapid heterologous expression with attenuated cytotoxicity in neurons.
Cited by
- Novel muscarinic receptor mutant mouse models.
- Engineered G-protein Coupled Receptors are Powerful Tools to Investigate Biological Processes and Behaviors
- Transmitting pain and itch messages: A contemporary view of the spinal cord circuits that generate Gate Control
- RiboTag is a flexible tool for measuring the translational state of targeted cells in heterogeneous cell cultures
- A thalamic input to the nucleus accumbens mediates opiate dependence
- Chemogenetic inhibition reveals midline thalamic nuclei and thalamo-accumbens projections mediate cocaine-seeking in rats
- Investigating metabolic regulation using targeted neuromodulation
- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely Tethered Ligands
- The Role of Astrocyte and Purinergic Signaling in RTN Chemoreception
- Intercellular Communication and Heterosynaptic Metaplasticity in the Rodent Hippocampus
- The molecular and cellular basis of cold sensation.
- Optogenetic Control of Hypocretin (Orexin) Neurons and Arousal Circuits
- From treatment to cure: stopping seizures, preventing seizures, and reducing brain propensity to seize.
- Activation of Ras and Rho GTPases and MAP Kinases by G-protein-coupled receptors.
- Changing channels in pain and epilepsy: Exploiting ion channel gene therapy for disorders of neuronal hyperexcitability
- Long-range synchrony between medial prefrontal cortex, thalamus and hippocampus underlies working memory behavior in mice.
- Regulation of the inhibitory drive in the olfactory bulb
- Oscillations dans la bande de fréquence gamma dans des modèles de rongeurs pour la schizophrénie
- Neural Circuit Mechanisms Underlying the Exacerbation of Alzheimer’s Disease by Chronic Stress
- A Method for Remotely Silencing Neural Activity in Rodents During Discrete Phases of Learning.
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