Phosphoprotein Analysis Using Antibodies Broadly Reactive against Phosphorylated Motifs*
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Summary
The substrates of most protein kinases remain unknown because of the difficulty tracing signaling pathways and identifying sites of protein phosphorylation, so a new approach to protein kinase substrate identification that combines immunoreactivity data with protein data base searches based upon antibody specificity is proposed.
- Type
- article
- Published
- 2002-10-18
- Cited by
- 285
- References
- 44
- Access
- Open access
- OpenAlex
- https://openalex.org/W1964211866
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:8129107
Keywords
Phosphoprotein, Phosphorylation, Antibody, Chemistry, Cell biology
References
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- Cell Biology: A Laboratory Handbook
- Signal transduction: Grabbing phosphoproteins
- Oncogenic kinase signalling
- Preparation and use of anti-phosphotyrosine antibodies to study structure and function of insulin receptor.
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- 3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase.
- Use of an oriented peptide library to determine the optimal substrates of protein kinases.
- Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae
- Comparison of the specificities of p70 S6 kinase and MAPKAP kinase‐1 identifies a relatively specific substrate for p70 S6 kinase: the N‐terminal kinase domain of MAPKAP kinase‐1 is essential for peptide phosphorylation
Cited by
- Anti‐phosphopeptide antibody, P‐STM as a novel tool for detecting mitotic phosphoproteins: Identification of lamins A and C as two major targets
- Enhancement of phosphoprotein analysis using a fluorescent affinity tag and mass spectrometry.
- Robust Enrichment of Phosphorylated Species in Complex Mixtures by Sequential Protein and Peptide Metal-Affinity Chromatography and Analysis by Tandem Mass Spectrometry
- Phosphoproteomics: new insights into cellular signaling
- Phospho‐proteomic analysis of cellular signaling
- Emerging applications for phospho-proteomics in cancer molecular therapeutics.
- Regulation of Akt/PKB by phosphatidylinositol 3‐kinase‐dependent and ‐independent pathways in B‐cell chronic lymphocytic leukemia cells: role of protein kinase Cβ
- Analytical strategies for phosphoproteomics
- Amino acid starvation induced by invasive bacterial pathogens triggers an innate host defense program.
- Generation of an immortalized mouse embryonic palatal mesenchyme cell line
- Selection of antigens for antibody-based proteomics
- Functional Proteomic Analysis of Lipid Raft Kinase Complexes
- Selective analysis of specific HLA ligand repertoires: poxviral CD8+ T cell epitopes and phosphorylated HLA ligands of tumor cells
- Dual function of LIMK2 in endothelial cells
- Enrichment strategy development for phosphoproteome analysis of saccharomyces cerevisiae
- Negative Ion Electron Capture Dissociation (niECD): A Novel Tandem Mass Spectrometric Technique.
- Identification of novel substrates of the energy-sensing kinase, AMP-activated protein kinase, and regulation of mTOR signaling during energy stress and mitosis
- Qualitative and quantitative analysis of post-translational modifications of chromatin in the mouse brain and sperm
- PhosphoPath: Visualization of Phosphosite-centric Dynamics in Temporal Molecular Networks.
- Rapid accumulation of Akt in mitochondria following phosphatidylinositol 3‐kinase activation
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