High-throughput analysis of yeast replicative aging using a microfluidic system
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Summary
An efficient, high-throughput microfluidic single-cell analysis chip in combination with high-resolution time-lapse microscopy that enables the determination of the yeast replicative lifespan in a high-Throughput manner and opens a new avenue for aging and longevity research using yeast genetic screens.
- Type
- article
- Published
- 2015-07-13
- Cited by
- 178
- References
- 49
- Access
- Open access
- OpenAlex
- https://openalex.org/W1949673751
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21982473
Keywords
Saccharomyces cerevisiae, Yeast, Throughput, Biology, Phenotype
References
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- Life Span of Individual Yeast Cells
- The rate of cell growth is governed by cell cycle stage.
- Sir2-Independent Life Span Extension by Calorie Restriction in Yeast
- Replicative and Chronological Aging in Saccharomyces cerevisiae
- Histone H4 lysine-16 acetylation regulates cellular lifespan
- Cell size and growth rate are major determinants of replicative lifespan
- End-of-life cell cycle arrest contributes to stochasticity of yeast replicative aging
- Logic of the Yeast Metabolic Cycle: Temporal Compartmentalization of Cellular Processes
- Transition metal homeostasis: from yeast to human disease
- Nervous yeast: modeling neurotoxic cell death.
- Tracking lineages of single cells in lines using a microfluidic device
- Shortest-Path Network Analysis Is a Useful Approach toward Identifying Genetic Determinants of Longevity
- Calorie restriction and prevention of age-associated chronic disease
- Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform
Cited by
- Advances in quantitative biology methods for studying replicative aging in Saccharomyces cerevisiae
- Yeast Replicator: a high-throughput multiplexed microfluidics platform for automated measurements of single-cell aging
- Soil-on-a-Chip: microfluidic platforms for environmental organismal studies.
- A Toolbox for Rapid Quantitative Assessment of Chronological Lifespan and Survival in Saccharomyces cerevisiae
- Biochemical analysis on microfluidic chips
- Microfluidic Technologies for Yeast Replicative Lifespan Studies
- Recent Progress of Microfluidics in Translational Applications
- High-Content Screening for Quantitative Cell Biology.
- Evidence for the hallmarks of human aging in replicatively aging yeast
- An aging-independent replicative lifespan in a symmetrically dividing eukaryote
- Microfluidic Platforms for Yeast-Based Aging Studies
- The Natural Variation in Lifespans of Single Yeast Cells Is Related to Variation in Cell Size, Ribosomal Protein, and Division Time
- The oxidation state of the cytoplasmic glutathione redox system does not correlate with replicative lifespan in yeast
- Budding Yeast: An Ideal Backdrop for In vivo Lipid Biochemistry
- Effects of an unusual poison identify a lifespan role for Topoisomerase 2 in Saccharomyces cerevisiae
- Microbial analysis by microencapsulation and its application to study proliferation heterogeneity.
- Morphologically Constrained and Data Informed Cell Segmentation of Budding Yeast
- Aggregation of an RNA-binding protein, not loss of heterochromatin, causes sterility of aging yeast cells
- High-throughput system-wide engineering and screening for microbial biotechnology.
- Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells.
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