Construction of Mutant Alleles in Saccharomyces cerevisiae without Cloning: Overview and the Delitto Perfetto Method

Keywords

Biology, Genetics, Mutagenesis, Locus (genetics), Saccharomyces cerevisiae

References

• Transformation of yeast with synthetic oligonucleotides.Rainer Hoenicke, Pam Tsai — 1988
• Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiaeCristiano Riciputi — 2009
• In vivo site-directed mutagenesis using oligonucleotidesFrancesca Storici, L. Kevin Lewis, Michael A. Resnick — 2001
• One-step gene disruption in yeast.Rodney Rothstein — 1983
• Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.Rodney Rothstein — 1991
• Site-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeastMisa Gray, Martin Kupiec, Saul M. Honigberg — 2004
• The delitto perfetto approach to in vivo site-directed mutagenesis and chromosome rearrangements with synthetic oligonucleotides in yeast.Francesca Storici, Michael A. Resnick — 2006
• A new efficient gene disruption cassette for repeated use in budding yeastUlrich Güldener — 1996
• Site-specific recombination determined by I-SceI, a mitochondrial group I intron-encoded endonuclease expressed in the yeast nucleus.Anne Plessis, Arnaud Perrin, James E. Haber — 1992
• Additional modules for versatile and economical PCR‐based gene deletion and modification in Saccharomyces cerevisiaeMark S. Longtine, Amos Mckenzie, Douglas J. DeMarini — 1998
• New vectors for combinatorial deletions in yeast chromosomes and for gap‐repair cloning using ‘split‐marker’ recombinationCécile Fairhead, Bertrand Llorente, Françoise Denis — 1996
• A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistanceJef D. Boeke, Francois La Croute, Gerald R. Fink — 1984
• Replacement of chromosome segments with altered DNA sequences constructed in vitro.Stephen W. Scherer, Ronald W. Davis — 1979
• Direct selection for gene replacement events in yeast.Kevin Struhl — 1983
• Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiaeAlan L. Goldstein, John H. McCusker — 1999
• Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.Linda Gritz, Julian Davies — 1983
• Construction of a marker gene cassette which is repeatedly usable for gene disruption in yeastAkio Toh‐e — 1995
• Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiaeSayoko Ito‐Harashima, John H. McCusker — 2004
• Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiaeAlan L. Goldstein, Xuewen Pan, John H. McCusker — 1999
• PCR‐mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiaeRinji Akada, Takao Kitagawa, Shohei Kaneko — 2006

Cited by

• The Genomic Landscape of Compensatory EvolutionBéla Szamecz, Gábor Boross, Dorottya Kalapis — 2014
• Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas.Andrew A. Horwitz, Jessica M. Walter, Max G. Schubert — 2015
• Gene editing for cell engineering: trends and applicationsSanjeev Kumar Gupta, Pratyoosh Shukla — 2017
• Simple CRISPR-Cas9 Genome Editing in S. cerevisiaeMarian F. Laughery, John J. Wyrick — 2019
• Emerging research topics detection with multiple machine learning modelsShuo Xu, Liyuan Hao, Xin An — 2019
• Opposing functions of the Hda1 complex and histone H2B mono-ubiquitylation in regulating cryptic transcription initiation in Saccharomyces cerevisiaeMargaret K. Shirra, Rachel A. Kocik, Mitchell A. Ellison — 2020
• Xrn1 is a deNADding Enzyme Modulating Mitochondrial NAD LevelsSunny Sharma, Jun Yang, Ewa Grudzien‐Nogalska — 2021
• Opposing functions of the Hda1 complex and histone H2B mono-ubiquitylation in regulating cryptic transcription in Saccharomyces cerevisiaeMargaret K. Shirra, Rachel A. Kocik, Mitchell A. Ellison — 2021
• Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNASunny Sharma, Jun Yang, Ewa Grudzien‐Nogalska — 2022
• Site-directed mutagenesis of Saccharomyces cerevisiae genome using mismatch PCR productMingtao Li, Xiaoyu You, Kunrong Mei — 2022
• Engineering and Evolution of the Complete Reductive Glycine Pathway in Saccharomyces cerevisiae for Formate and CO2 AssimilationViswanada R Bysani, A. S. M. Towhidul Alam, Arren Bar‐Even — 2023
• Yeast Genome Mutagenesis With Multi‐Mismatch PCR: A Rapid and Efficient Strategy for Site‐Directed Mutagenesis in Saccharomyces cerevisiaeShuaihua Cao, Yixin Ding, Mingtao Li — 2025

Related papers

• Trp, Thr Analogue 복합 저항성 Saccharomyces cerevisiae 균주 개발염형준, 이승현, 김선혜 — 2004
• Isolation of Trp, Thr Overproducing Strain of Saccharomyces cerevisiae염형준, 이승현, 김선혜 — 2004
• Allele-mining of rice blast resistance genes at AC134922 locus.Dan Wang, Changjiang Guo, Ju Huang — 2014
• SUM1, an apparent positive regulator of the cryptic mating-type loci in Saccharomyces cerevisiae.Amar J. S. Klar, Satya N Kakar, John M. Ivy — 1985
• Characterisation of genetic control of thermotolerance in mutants of Saccharomyces cerevisiaeLeela Wati, S. S. Dhamija, Deeksha Singh - — 1996
• Breeding of High-yield GABA Saccharomyces Cerevisiae Strain by NTG MutagenesisLihua Huang, Hu Chao, Bin Zuo — 2010
• Genetics of Zoarces populations. I. Three loci determining the phosphoglucomutase isoenzymes in brain tissue.J. Peter Hjorth — 2009
• Classical and molecular genetic analyses of his-3 mutants of Neurospora crassa. II. Southern blot analyses and molecular mechanisms of mutagenicity.Jeffrey S. Dubins, Laurie K. Overton, Ronald R. Cobb — 1989
• Localized mutagenesis in Streptomyces coelicolor A3 (2).Rosalba Randazzo, Giulia Sciandrello, A. Carere — 1976