Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae
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Summary
Three new dominant drug resistance cassettes have unique antibiotic resistance phenotypes and do not affect growth when inserted into the ho locus, which make the cassettes ideally suited for creating S. cerevisiae strains with multiple mutations within a single strain.
- Type
- article
- Published
- 1999-10-01
- Cited by
- 1,970
- References
- 45
- Access
- Open access
- OpenAlex
- https://openalex.org/W1997859335
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:35505337
Keywords
Biology, Plasmid, Hygromycin B, Genetics, Kanamycin
References
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- Exploring redundancy in the yeast genome: an improved strategy for use of the cre-loxP system.
- Inhibition of climbing fibres is a signal for the extinction of conditioned eyelid responses
- CRR1, a gene encoding a putative transglycosidase, is required for proper spore wall assembly in Saccharomyces cerevisiae.
- The HEAT repeat protein Blm10 regulates the yeast proteasome by capping the core particle
- Azo Reductase Activity of Intact Saccharomyces cerevisiae Cells Is Dependent on the Fre1p Component of Plasma Membrane Ferric Reductase
- Localization of telomeres and telomere-associated proteins in telomerase-negative Saccharomyces cerevisiae
- Degradation of a Cytosolic Protein Requires Endoplasmic Reticulum-associated Degradation Machinery*
- The NatA acetyltransferase couples Sup35 prion complexes to the [PSI+] phenotype.
- Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae
- Nourseothricin acetyltransferase: a new dominant selectable marker for the dermatophyte Trichophyton mentagrophytes.
- Chromosome rearrangements and aneuploidy in yeast strains lacking both Tel1p and Mec1p reflect deficiencies in two different mechanisms
- The ATPase activity of Fml1 is essential for its roles in homologous recombination and DNA repair
- Insulin-induced Gene Protein (INSIG)-dependent Sterol Regulation of Hmg2 Endoplasmic Reticulum-associated Degradation (ERAD) in Yeast*
- Eukaryotic Oligosaccharyltransferase Generates Free Oligosaccharides during N-Glycosylation*
- The Role of Exo1p Exonuclease in DNA End Resection to Generate Gene Conversion Tracts in Saccharomyces cerevisiae
- Cdc42p-Interacting Protein Bem4p Regulates the Filamentous-Growth Mitogen-Activated Protein Kinase Pathway
- Control of Plasma Membrane Permeability by ABC Transporters
- Temporal Expression Profiling Identifies Pathways Mediating Effect of Causal Variant on Phenotype
- Probiotic Saccharomyces cerevisiae strains as biotherapeutic tools: is there room for improvement?
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