Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single-Tube Multiplex Reverse Transcription-PCR: Feasibility Study
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Summary
The results show that the multiplex RT-PCR–enzyme-linked immunosorbent assay is a useful and rapid diagnostic tool for the management of children with ARI and studies of the overall benefit of this method with regard to the use of antibiotics, theUse of diagnostic procedures including additional microbiological tests, and hospitalization rate and duration are warranted.
- Type
- article
- Published
- 1999-01-01
- Cited by
- 266
- References
- 40
- Access
- Open access
- OpenAlex
- https://openalex.org/W1907972820
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:40260605
Keywords
Mycoplasma pneumoniae, Multiplex polymerase chain reaction, Enterovirus, Virology, Multiplex
References
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- Identification of Chlamydia pneumoniae by DNA amplification of the 16S rRNA gene
- Simultaneous Detection and Identification of Human Parainfluenza Viruses 1, 2, and 3 from Clinical Samples by Multiplex PCR
- Genus Level Identification of Mycobacteria from Clinical Specimens by Using an Easy-To-Handle Mycobacterium-Specific PCR Assay
- Detection of adenovirus in clinical specimens by polymerase chain reaction and liquid-phase hybridization quantitated by time-resolved fluorometry
- Multiplex reverse transcription-PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
- Application of a Nested, Multiplex PCR to Psittacosis Outbreaks
- Bacterial antibody assays in the diagnosis of acute lower respiratory tract infection in children.
- Effect of rapid viral diagnosis on the management of children hospitalized with lower respiratory tract infection.
- Dual Respiratory Virus Infections
- Failure to detect Chlamydia pneumoniae in ear fluids from children with otitis media.
- Community-acquired pneumonia
- Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction
- Pediatric hospitalizations for croup (laryngotracheobronchitis): biennial increases associated with human parainfluenza virus 1 epidemics.
Cited by
- Multiplex PCR for rapid and differential diagnosis of Mycoplasma pneumoniae and Chlamydia pneumoniae in respiratory infections.
- Multiplex PCR: Optimization and Application in Diagnostic Virology
- RSV-Prevention in Children Guided by a Web-Based Early Warning System
- Development of Real-Time Multiplex Nucleic Acid Sequence-Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. in Respiratory Specimens
- Co-Infections with Influenza and Other Respiratory Viruses
- Evaluation and adaptation of molecular approaches for detection and characterization of viruses of the respiratory tract
- Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogens
- Rapid detection of bacterial atypical pneumonia agents by multiplex PCR.
- Analyse du polymorphisme associé aux répétitions en tandem pour le typage de deux espèces de mycoplasmes pathogènes chez l’homme : mycoplasma genitalium et Mycoplasma pneumoniae
- Societal implications of nanoscience and nanotechnology
- Identification of mollicutes and characterisation of Mycoplasma hyopneumoniae isolates
- Prevalência de vírus respiratórios em crianças de creche com sintomas de infecções respiratórias agudas
- Molecular analysis of adenoviruses from clinical samples
- Diagnostic value of brochoalveolar lavage in infectious disorders
- Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays
- Activation of cytokines and NF-kappa B in corneal epithelial cells infected by respiratory syncytial virus: potential relevance in ocular inflammation and respiratory infection
- Microbial aetiology of community acquired pneumonia at a tertiary institution in Johannesburg, South Africa
- Human adenovirus type 8 epidemic keratoconjunctivitis with large corneal epithelial full-layer detachment: an endemic outbreak with uncommon manifestations
- Laboratory diagnosis of Mycoplasma pneumoniae infection.
- The role of DNA amplification technology in the diagnosis of infectious diseases.
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