Multiplex PCR: Optimization and Application in Diagnostic Virology
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Summary
This article reviews the principles, optimization, and application of multiplex PCR for the detection of viruses of clinical and epidemiological importance and the use of hot start-based PCR methodology.
- Type
- article
- Published
- 2000-10-01
- Cited by
- 927
- References
- 130
- OpenAlex
- https://openalex.org/W11023957
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:18507974
Keywords
Priming (agriculture), Psychology, Neuroscience, Biology, Botany
References
- Development of a multiplex PCR assay for the simultaneous detection and discrimination of HIV-1, HIV-2, HTLV-I and HTLV-II.
- Multiplex PCR: critical parameters and step-by-step protocol.
- Multiplex detection of four pathogenic retroviruses using molecular beacons.
- Combining multiplex and touchdown PCR to screen murine microsatellite polymorphisms.
- Diagnosis of cytomegalovirus pneumonia by the polymerase chain reaction with archived frozen lung tissue and bronchoalveolar lavage fluid.
- An introduction to PCR primer design and optimization of amplification reactions.
- Rapid simultaneous detection of multiple retroviral DNA sequences using the polymerase chain reaction and capillary DNA chromatography.
- Multiplex PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Genitourinary specimens
- Genotyping by multiplex polymerase chain reaction for detection of endemic hepatitis B virus transmission
- Detection of hog cholera virus and differentiation from other pestiviruses by polymerase chain reaction
- Differentiation of Campylobacter jejuni and Campylobacter coli by polymerase chain reaction.
- PCR protocols — A guide to methods and applications
- Advances in nucleic acid-based detection methods
- Selecting Optimal Oligonucleotide Primers for Multiplex PCR
- Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory
- Simultaneous Detection and Identification of Human Parainfluenza Viruses 1, 2, and 3 from Clinical Samples by Multiplex PCR
- Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single-Tube Multiplex Reverse Transcription-PCR: Feasibility Study
- Multicenter international work flow study of an automated polymerase chain reaction instrument.
- Multiplex reverse transcription-PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
- Multiplex PCR for Typing and Subtyping Influenza and Respiratory Syncytial Viruses
Cited by
- Development of improved diagnostic testing methods for invasive pneumococcal disease
- Microarray-based detection and genotyping of viral pathogens
- Optimization of fragmentation conditions for microarray analysis of viral RNA.
- Microarray-based detection of bacteria by on-chip PCR.
- A Study on viral etiologies of lower respiratory infections and molecular characterization of influenza virus H1N1 2009 circulating in central Vietnam
- High prevalence, low counts and uncommon serotypes of Listeria monocytogenes in linguiça, a Brazilian fresh pork sausage.
- Multiplex real-time PCR for identification of canine parvovirus antigenic types.
- Amplification chemistries in clinical virology
- Detecção de vírus implicados em infecções respiratórias e avaliação da sua diversidade em amostras clínicas
- Strangles: The Molecular Identification and Epidemiology of Streptococcus equi subsp. equi
- The evaluation of multiplex PCR and DNA profiling methods (DGGE and SSCP) for the detection of mycotoxigenic Fusarium species
- Massively parallel DNA sequencing: the new frontier in biogeography
- Applications of viral metagenomics in the veterinary field
- An introduction to DNA technologies and their role in livestock production: a review.
- Guidelines for the qualitative detection of viral genomes in dried blood spots.
- Enhancing Transplant Safety: A New Era in the Microbiologic Evaluation of Organ Donors?
- DEVELOPMENT OF MOLECULAR DIAGNOSTIC ASSAYS FOR EQUINE RESPIRATORY VIRUSES AND ANALYSIS OF THE ROLE OF EQUINE ARTERITIS VIRUS ENVELOPE PROTEINS IN THE EARLY EVENTS OF VIRUS ENTRY
- Development of molecular techniques for fungal diagnostic research
- Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization
- ESCHERICHIA COLI O157: DETECTION AND QUANTIFICATION IN CATTLE FECES BY QUANTITATIVE PCR, CONVENTIONAL PCR, AND CULTURE METHODS
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