Rapid procedure for detection and isolation of large and small plasmids

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Summary

The method utilized the molecular characteristics of covalently closed circular deoxyribonucleic acid (DNA) released from cells under conditions that denature chromosomal DNA by using alkaline sodium dodecyl sulfate (pH 12.6) at elevated temperatures.

Type
article
Published
1981-03-01
Cited by
3,055
References
29
Access
Open access

Keywords

Biology, Plasmid, DNA, Alkaline lysis, Plasmid preparation

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