Rapid procedure for detection and isolation of large and small plasmids
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Summary
The method utilized the molecular characteristics of covalently closed circular deoxyribonucleic acid (DNA) released from cells under conditions that denature chromosomal DNA by using alkaline sodium dodecyl sulfate (pH 12.6) at elevated temperatures.
- Type
- article
- Published
- 1981-03-01
- Cited by
- 3,055
- References
- 29
- Access
- Open access
- OpenAlex
- https://openalex.org/W1690413094
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:1690134
Keywords
Biology, Plasmid, DNA, Alkaline lysis, Plasmid preparation
References
- Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
- Plasmid deoxyribonucleic acid in Rhizobium vigna and Rhizobium trifolii.
- Apparent Involvement of a Plasmid in Phaseotoxin Production by Pseudomonas phaseolicola
- Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5
- General Method for the Isolation of Plasmid Deoxyribonucleic Acid
- Detection and characterization of plasmids in Pseudomonas glycinea
- Physical properties and gel electrophoresis behavior of R12-derived plasmid DNAs.
- Novel screening procedure for recombinant plasmids.
- Concentration and desalting of ribonucleases.
- Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid
- Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.
- A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
- A freeze-squeeze method for recovering long DNA from agarose gels.
- A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria.
- Volume changes accompanying thermal denaturation of deoxyribonucleic acid. II. Denaturation at alkaline pH
- Isolation of covalently closed circular DNA of high molecular weight from bacteria.
- Alkaline denaturation of DNA's from various sources.
- Isolation of large molecular weight DNA from agarose gels forfurther digestion by restriction enzymes
- A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.
- Identification and Characterization of Large Plasmids in Rhizobium meliloti using Agarose Gel Electrophoresis
Cited by
- Growth phase and SpvR regulation of transcription of Salmonella typhimurium spvABC virulence genes.
- Biochemical and molecular characterization of Salmonella enterica serovar berta, and comparison of methods for typing
- Oxygen resistant strain of N2-fixing Escherichia coli.
- Methods for studying bacterial gene transfer in soil by conjugation and transduction.
- Purification of RNA-free plasmid DNA using alkaline extraction followed by Ultrogel A2 column chromatography.
- Acquisition of a drug resistance plasmid converts Salmonella enteritidis phage type 4 to phage type 24
- CS31A, a new K88-related fimbrial antigen on bovine enterotoxigenic and septicemic Escherichia coli strains
- Electroporation-induced transformation of intact cells of Clostridium perfringens
- Tn5-mediated integration of the delta-endotoxin gene from Bacillus thuringiensis into the chromosome of root-colonizing pseudomonads
- Plasmid deoxyribonucleic acid in throat and cerebrospinal fluid isolates of Neissepia meningitidis
- Children with trimethoprim- and ampicillin-resistant fecal Escherichia coli in day care centers.
- Mycobacterial Plasmids
- Conservation of plasmids among plant-pathogenic Pseudomonas syringae isolates of diverse origins.
- Escherichia coli K12 strains for use in the identification and characterization of colicins.
- Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis
- Plasmid-mediated degradation of dibenzothiophene by Pseudomonas species
- Mode of infection, nodulation specificity, and indigenous plasmids of 11 fast-growing Rhizobium japonicum strains
- Thermophilic anaerobic bacteria which ferment hemicellulose: characterization of organisms and identification of plasmids
- Molecular characterization and antibiotic susceptibility of Vibrio cholerae non-O1
- Genetic and molecular characterization of capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 3
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