Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction
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Summary
The application of reverse micellar extraction for the separation of chromosomal from plasmid DNA is described and it is shown that by applying different procedures for alkaline lysis, bacterial lysates with different amounts of chromosome DNA were generated.
- Type
- article
- Published
- 2009-06-27
- Cited by
- 4
- References
- 21
- OpenAlex
- https://openalex.org/W1981289100
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10542739
Keywords
Alkaline lysis, Plasmid, Plasmid preparation, Lysis, DNA
References
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- A Laboratory manual
- Surfactant - Based Separation Processes
- The impact of fluid-dynamic-generated stresses on chDNA and pDNA stability during alkaline cell lysis for gene therapy products.
- Biochemical engineering approaches to the challenges of producing pure plasmid DNA.
- A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
- Alkaline‐cell lysis through in‐line static mixer reactor for the production of plasmid DNA for gene therapy
- Supercoiled plasmid DNA: selective purification by thiophilic/aromatic adsorption.
- Integration of Bacteria Capture via Filtration and in Situ Lysis for Recovery of Plasmid DNA under Industry‐Compatible Conditions
- Extraction of DNA by Reversed Micelles
- Isolierung von Plasmid-DNA durch inversmizellare Zweiphasensysteme : Optimierung der Rückextraktion
- Proposal for a better integration of bacterial lysis into the production of plasmid DNA at large scale.
- Reverse micellar extraction systems for the purification of pharmaceutical grade plasmid DNA.
- Prediction of Shear Damage of Plasmid DNA in Pump and Centrifuge Operations Using an Ultra Scale‐Down Device
- Production of supercoiled multimeric plasmid DNA for biopharmaceutical application.
- Downstream Processing of Plasmid DNA for Gene Therapy and Genetic Vaccination
- Molecular Cloning: A Laboratory Manual
- Degradation of supercoiled plasmid DNA within a capillary device
- Production of plasmid DNA for pharmaceutical use.
- Mechanical disruption of Escherichia coli for plasmid recovery.
Cited by
- Comparison of Alkaline Lysis with Electroextraction and Optimization of Electric Pulses to Extract Plasmid DNA from Escherichia coli
- Genetic variation of Sporothrix globosa isolates from diverse geographic and clinical origins in China
- Optimized plasmid extraction through controlled temperature, prolonged alkaline lysis, and gentle mixing.
- Optimized Plasmid Extraction Through Controlled Temperature, Prolonged Alkaline Lysis, and Gentle Mixing
- Pulsed Electrical Discharges: Principles and Application to Extraction of Biocompounds
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