Alignment of cryo-EM movies of individual particles by optimization of image translations.
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Summary
An algorithm is described that allows for individual <1 MDa particle images to be aligned without frame averaging or linear trajectories, and can be used to improve 3-D maps from single particle cryo-EM.
- Type
- preprint
- Published
- 2014-09-24
- Cited by
- 369
- References
- 15
- Access
- Open access
- OpenAlex
- https://openalex.org/W1686036901
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:17501197
Keywords
Particle (ecology), Weighting, Single particle analysis, Reference frame, Physics
References
- Beam-Induced Motion of Vitrified Specimen on Holey Carbon Film
- A Limited Memory Algorithm for Bound Constrained Optimization
- Molecular structure determination by electron microscopy of unstained crystalline specimens.
- Electron microscope imaging of single particles using the Medipix2 detector
- Images of paraffin monolayer crystals with perfect contrast: minimization of beam-induced specimen motion
- Radiation damage of purple membrane at low temperature.
- The resolution dependence of optimal exposures in liquid nitrogen temperature electron cryomicroscopy of catalase crystals.
- Tilt-Pair Analysis of Images from a Range of Different Specimens in Single-Particle Electron Cryomicroscopy
- RELION: Implementation of a Bayesian approach to cryo-EM structure determination
- 2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy
- Beam-induced motion correction for sub-megadalton cryo-EM particles
- Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6
- Electron counting and beam-induced motion correction enable near atomic resolution single particle cryoEM
- Structure of the TRPV1 ion channel determined by electron cryo-microscopy
- Faculty Opinions recommendation of TRPV1 structures in distinct conformations reveal activation mechanisms.
Cited by
- Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV.
- Automatic estimation and correction of anisotropic magnification distortion in electron microscopes.
- Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase
- A Primer to Single-Particle Cryo-Electron Microscopy
- Structural Insights into KCTD Protein Assembly and Cullin3 Recognition.
- Progress towards an optimal specimen support for electron cryomicroscopy
- Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
- Single-particle cryo-EM data acquisition by using direct electron detection camera.
- Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM
- High Resolution Single Particle Refinement in EMAN2.1
- An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology
- Models for the a subunits of the Thermus thermophilus V/A-ATPase and Saccharomyces cerevisiae V-ATPase enzymes by cryo-EM and evolutionary covariance
- Fundamental Limits in Multi-Image Alignment
- Methods to account for movement and flexibility in cryo-EM data processing
- Structural basis for gating and activation of RyR1
- Box C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs
- Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study
- The structural basis of modified nucleosome recognition by 53BP1
- Anisotropic Correction of Beam-induced Motion for Improved Single-particle Electron Cryo-microscopy
- Cryo-EM studies of the structure and dynamics of vacuolar-type ATPases
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