Proteomic analysis of the Escherichia coli outer membrane.
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Summary
This research readily demonstrates that integral OMPs, commonly missing from 2D gel maps, are amenable to separation by two-dimensional electrophoresis and shown the value of parallel protein analysis to document changes in E. coli OMP expression as influenced by culture temperature.
- Type
- article
- Published
- 2000-05-01
- Cited by
- 485
- References
- 33
- Access
- Open access
- OpenAlex
- https://openalex.org/W1548038775
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:11369437
Keywords
Bacterial outer membrane, Escherichia coli, Biology, Bacteria, ORFS
References
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- Extraction of membrane proteins by differential solubilization for separation using two‐dimensional gel electrophoresis
- Antigen 43, a phase-variable bipartite outer membrane protein, determines colony morphology and autoaggregation in Escherichia coli K-12.
- Towards an automated approach for protein identification in proteome projects
- Bacterial porins: structure and function.
- Improved high-performance liquid chromatography of amino acids derivatised with 9-fluorenylmethyl chloroformate
- Extraction of Escherichia coli proteins with organic solvents prior to two‐dimensional electrophoresis
- Immunological comparison of major outer membrane proteins from different strains of Escherichia coli.
- Outer membrane protein 3B of Escherichia coli K-12: Effects of growth temperature on the amount of the protein and further characterization on acrylamide gels
- Two-dimensional gel electrophoresis of membrane proteins.
- Antibodies to Escherichia coli 06 porins cross-react with urinary pathogens.
- Generic assignments, strain histories, and properties of pure cultures of cyanobacteria
- Electroeluted outer membrane proteins as immunogens.
- Macrophages recognize and adhere to an OmpD-like protein of Salmonella typhimurium.
- General and specific porins from bacterial outer membranes.
Cited by
- Application of proteomics to Pseudomonas aeruginosa.
- Studies on Methylmalonyl-CoA Mutase from Escherichia coli
- Complementing genomics with proteomics: The membrane subproteome of Pseudomonas aeruginosa PAO1
- The SlyB outer membrane lipoprotein of Burkholderia multivorans contributes to membrane integrity.
- The cell-envelope proteome of Bifidobacterium longum in an in vitro bile environment.
- Enzymatic, outer membrane proteins and plasmid alterations of starved Vibrio parahaemolyticus and Vibrio alginolyticus cells in seawater
- A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions
- Proteomic tools for environmental microbiology—A roadmap from sample preparation to protein identification and quantification
- Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus
- Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T.
- Isolation and solubilization of cellular membrane proteins from bacteria.
- Caracterización estructural de bacterias antárticas adaptadas al frío y detección de nuevos emulsionantes: estudio de la cepa "Shewanella vesiculosa M7(T)”
- In-gel proteomics : optimisation of Coomassie Brilliant Blue staining as a fluorescent alternative for sensitive protein detection
- Proteomic analysis of the biofilm and biofilm-associated phenotypes of Pseudomonas aeruginosa cultured in batch
- Iron Acquisition by Uropathogenic Escherichia coli: ChuA and Hma Heme Receptors as Virulence Determinants and Vaccine Targets.
- Vitamin E (Tocotrienols) and Prostate Cancer: A Proteomics Approach.
- Interactions between oral biofilm bacteria, Streptococcus mutans and Veillonella parvula
- Identification of the outer membrane immunoproteome of the uropathogen Proteus mirabilis: Insights into virulence and potential vaccine candidates
- Isolation of bacterial cell membranes proteins using carbonate extraction.
- Analysis of bacterial proteins by 2DE.
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