Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells.
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Summary
The general utility of the baculovirus system for production of large quantities of native G protein-coupled receptors is demonstrated, consistent with its known selectivity for pertussis toxin-insensitive G proteins ("Gp").
- Type
- article
- Published
- 1991-01-05
- Cited by
- 257
- References
- 4
- Access
- Open access
- OpenAlex
- https://openalex.org/W1510104405
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:5540381
Keywords
Insect, Receptor, Biology, Cell biology, Virology
References
Cited by
- The use of the amplifiable high-expression vector pEE14 to study the interactions of autoantibodies with recombinant human thyrotrophin receptor.
- Photoaffinity labeling of the human brain cholecystokinin receptor overexpressed in insect cells. Solubilization, deglycosylation and purification.
- Desensitization and Sequestration of Human m2 Muscarinic Acetylcholine Receptors by Autoantibodies from Patients with Chagas’ Disease*
- Interaction of the D2short dopamine receptor with G proteins: analysis of receptor/G protein selectivity.
- Biotechnology of β-adrenergic receptors
- Protein interaction assays with G proteins.
- Quantitative assays for GTPase-activating proteins.
- Assay of RGS protein activity in vitro using purified components.
- Roles of Muscarinic Receptors m1 and m2 on Interleukin 2 Production in Human Peripheral Blood Lymphocytes and Human Leukemic T Cell Line Jurkat Cells
- The Pharmacology of Functional, Biochemical, and Recombinant Receptor Systems
- Viral Expression Vectors
- Regulation of G protein-coupled receptor kinase 2.
- Differential Activity of the G Protein β5γ2 Subunit at Receptors and Effectors*
- Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C
- Expression of G protein-coupled receptors and G proteins in Sf9 cells: analysis of coupling by radioligand binding.
- Expression of the Extracellular Domain of the Thyrotropin Receptor in the Baculovirus System Using a Promoter Active Earlier than the Polyhedrin Promoter
- Analysis of altered G-protein subunit accumulation in Cryphonectria parasitica reveals a third Gα homologue
- Dynamic palmitoylation of G-protein-coupled receptors in eukaryotic cells.
- Purification of Recombinant Porcine m2 Muscarinic Acetylcholine Receptor from Chinese Hamster Ovary Cells
- Dominant-negative Inhibition of Pheromone Receptor Signaling by a Single Point Mutation in the G Protein α Subunit*
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