Processive translocation and DNA unwinding by individual RecBCD enzyme molecules

Keywords

RecBCD, Helicase, DNA, Biophysics, Base pair

References

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• Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins.John E. Cronan — 1990
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• Chi‐activated RecBCD enzyme possesses 5′→3′ nucleolytic activity, but RecBC enzyme does not: evidence suggesting that the alteration induced by Chi is not simply ejection of the RecD subunitDaniel G. Anderson, Jason Churchill, Stephen C. Kowalczykowski — 1997
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• Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Dan A. Dixon, Jason Churchill, S C Kowalczykowski — 1994
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• Differences in the chemical reactivity of individual molecules of an enzymeQifeng Xue, Edward S. Yeung — 1995
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• A model for the mechanism of polymerase translocation.Richard J. Guajardo, Rui Sousa — 1997
• Interaction with the recombination hot spot chi in vivo converts the RecBCD enzyme of Escherichia coli into a chi-independent recombinase by inactivation of the RecD subunit.Arjen Koppen, Sylvia Krobitsch, Brigitte Thoms — 1995

Cited by

• Single-molecule studies of complex systems: the replisome.Dian Savitri — 2007
• Real-time direct observation of single-molecule DNA hydrolysis by exonucleaseIIIHirofumi Kurita, Ken-ichi Inaishi, Ken Torii — 2006
• Mobility analysis of super-resolved proteins on optically stretched DNA: comparing imaging techniques and parameters.Nuwan Jayawickreme — 2014
• Sequence-dependent nanometer-scale conformational dynamics of individual RecBCD–DNA complexesLionel Amatte — 2016
• Integrating single-molecule visualization and DNA micromanipulationJoost van Mameren — 2002
• Single-Molecule Investigations of Enzyme Dynamics: RecBCD Helicase Motion and glmS Ribozyme CleavageM. Hosotani — 2011
• Kinetics of Motor Protein Translocation on Single Stranded DNAChristopher J. Fischer, A. Lake Wooten, Eric J. Tomko — 2010
• DNA curtains: novel tools for imaging protein-nucleic acid interactions at the single-molecule level.Bridget E. Collins, Ling F. Ye, Daniel Duzdevich — 2014
• Single-molecule and single-particle imaging of molecular motors in vitro and in vivo.Natalia Fili — 2014
• Structure and Mechanisms of SF2 DNA Helicases.David C. Beyer, Mohamed Ghoneim, Maria Spies — 2013
• Overview: what are helicases?Colin G. Wu, Maria Spies — 2013
• Fluorescence and labelling: how to choose and what to do.Natalia Fili, Christopher P. Toseland — 2014
• Orf protein modulates phage and bacterial pathways of genetic recombinationLaura Y. Bowers — 2008
• The fluorescence properties and binding mechanism of SYTOX green, a bright, low photo-damage DNA intercalating agentShreyasi Thakur, Diego I. Cattoni, Marcelo Nöllmann — 2015
• Ten years of tension: single-molecule DNAZev Bryant, Steven B. Smith — 2003
• DNA Helicases and DNA Motor ProteinsMaria Spies — 2013
• Fluorescent Methods for Molecular MotorsChristopher P. Toseland, Natalia Fili — 2014
• PURIFICATION AND CHARACTERIZATION OF THE RECD PROTEIN-HOMOLOGUE FROM DEINOCOCCUS RADIODURANSJianlei Wang — 2004
• Identification of pre-synaptic processing proteins from bacteroides fragilisFrances Parry — 2011
• Optical diffraction-based silicon sensors for the detection of DNA sequencesA. Weld — 2007

Related papers

• RecBCD enzyme is a bipolar DNA helicaseMark S. Dillingham, Maria Spies, Stephen C. Kowalczykowski — 2003
• Small-molecule sensitization of RecBCD helicase-nuclease to a Chi hotspot-activated stateAhmet Karabulut, Ryan T. Cirz, Gerald R. Smith — 2020
• Monitoring the Individual Motor Activities of RecB and RecD within the E.coli Recbcd HelicaseFuqian Xie — 2011
• Small-molecule sensitization of RecBCD helicase–nuclease to a Chi hotspot-activated stateAhmet Karabulut, Ryan T. Cirz, Andrew F. Taylor — 2020
• RecBCD enzyme is a DNA helicase with fast and slow motors of opposite polarityAndrew F. Taylor, Gerald R. Smith — 2003
• Direct observation of RecBCD helicase as single-stranded DNA translocases.Cinya Chung, Hung‐Wen Li — 2013
• Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.Susan K. Amundsen, Andrew F. Taylor, Manjula Reddy — 2007
• Direct Observation of Recbcd Helicase as ssDNA TranslocasesCinya Chung, Hung‐Wen Li — 2013
• A model of DNA unwinding dynamics by the RecBCD complex and its regulation by Chi recognition.Ping Xie — 2018