Safer derivatives of bacteriophage λgt.λC for use in cloning of recombinant DNA molecules
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Summary
A mutant strain of bacteriophage λ (λgt.λC) is constructed which is especially well suited to studies involving the cloning of recombinant DNA molecules, and has only two EcoRI restriction endonuclease sites which reduce the phage chromosome to three DNA fragments.
- Type
- article
- Published
- 1976-02-19
- Cited by
- 64
- References
- 10
- OpenAlex
- https://openalex.org/W1502538436
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4163724
Keywords
Bacteriophage, Recombinant DNA, EcoRI, In vitro recombination, Restriction enzyme
References
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- Properties of a mutant of Escherichia coli defective in bacteriophage lambda head formation (groE). I. Initial characterization.
- Bacteriophage T4 transfer RNA. I. Isolation and characterization of two-phage-coded nonsense suppressors.
- The Bacteriophage Lambda.
- Analysis of Endonuclease R·EcoRI Fragments of DNA from Lambdoid Bacteriophages and Other Viruses by Agarose-Gel Electrophoresis
- Thermal asymmetry of site-specific recombination by bacteriophage lambda.
- Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA.
- New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.
- Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments
- Asilomar conference on recombinant DNA molecules.
Cited by
- Use of recombinant DNA technology for the production of polypeptides.
- Sequence comparison in the crossover region of an oncogenic avian retrovirus recombinant and its nononcogenic parent: genetic regions that control growth rate and oncogenic potential
- Variance of sequence divergence.
- Isolation and characterization of a ColE1 plasmid containing the entire Bio gene cluster of Escherichia coli K12
- Identification of the uvrD gene product of Salmonella typhimurium LT2
- Construction of a hybrid bacteriophage-plasmid recombinant DNA vector
- Structural organization and biological activity of molecular clones of the integrated genome of a BALB/c mouse sarcoma virus
- Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning
- Aspects of genetic engineering in micro-organisms.
- Molecular cloning of integrated Gardner-Rasheed feline sarcoma virus: genetic structure of its cell-derived sequence differs from that of other tyrosine kinase-coding onc genes
- Intramolecular integration within Moloney murine leukemia virus DNA
- Cloning and mapping of the manganese superoxide dismutase gene (sodA) of Escherichia coli K-12
- Survival of Escherichia coli host-vector systems in the mammalian intestine.
- lambdaplac5 derivatives, potential vectors for DNA fragments cleaved by Streptomyces stanfordii restriction enzyme (SstI).
- Modulation of the initiation of mouse beta-globin transcription by non-histone proteins purified from mouse erythropoietic Friend cells.
- A simple technique for the isolation of deletion mutants of phage lambda.
- Identification of transcription initiation sites for bacterial RNA polymerase and eukaryotic RNA polymerase B on the 5' end of the mouse beta-Globin gene.
- Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
- Rat kappa-chain J-segment genes: two recent gene duplications separate rat and mouse.
- Tissue-specific formation of transcription-initiation complexes at the 5' end of the mouse beta major globin gene.
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