Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop.
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Summary
The results demonstrate the potential for detection of low-level autoreactive T cell responses and identify assay characteristics that will be useful for studies in type 1 diabetes.
- Type
- article
- Published
- 2003-12-01
- Cited by
- 86
- References
- 28
- OpenAlex
- https://openalex.org/W1500301225
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:25396193
Keywords
ELISPOT, Immunology, Antigen, Toxoid, Ex vivo
References
- Redirection of human autoreactive T-cells Upon interaction with dendritic cells modulated by TX527, an analog of 1,25 dihydroxyvitamin D(3).
- Recurrent diabetes mellitus in the pancreas iso- and allograft. A light and electron microscopic and immunohistochemical analysis of four cases.
- Single-Cytokine-Producing CD4 Memory Cells Predominate in Type 1 and Type 2 Immunity1
- Development of type 1 diabetes despite severe hereditary B-cell deficiency.
- Decreased In Vitro and IL-10 Production by Peripheral Blood in First Degree Relatives at High Risk of Diabetes Type-I
- A comparison between ELISPOT methods for the detection of cytokine producing cells: greater sensitivity and specificity using ELISA plates as compared to nitrocellulose membranes.
- Transfer of insulin-dependent diabetes between HLA-identical siblings by bone marrow transplantation.
- A disease-associated cellular immune response in type 1 diabetics to an immunodominant epitope of insulin.
- Autoreactive T cell responses in insulin-dependent (Type 1) diabetes mellitus. Report of the first international workshop for standardization of T cell assays.
- Activated CD4+ and CD8+ T‐lymphocytes in Newly Diagnosed Type 1 Diabetes: a Prospective Study
- Th1-like dominance in high-risk first-degree relatives of Type I diabetic patients
- Increase in Simultaneous Coexpression of Naive and Memory Lymphocyte Markers at Diagnosis of IDDM
- Decreased secretion of Th2 cytokines precedes Up-regulated and delayed secretion of Th1 cytokines in activated peripheral blood mononuclear cells from patients with insulin-dependent diabetes mellitus.
- Autoreactive T cells in endocrine/organ-specific autoimmunity: why has progress been so slow?
- Increased CD69 and human leukocyte antigen-DR expression on T lymphocytes in insulin-dependent diabetes mellitus of long standing.
- Detection of GAD65-reactive T-Cells in type 1 diabetes by immunoglobulin-free ELISPOT assays.
- Increased numbers of in vivo activated T cells in patients with recent onset insulin-dependent diabetes mellitus.
- A T Cell Clone’s Avidity Is a Function of Its Activation State1
- Cytokine detection by ELISPOT: relevance for immunological studies in type 1 diabetes
- Induction of Interferon-γ and IL-4 Production by Mitogen and Specific Antigens in Peripheral Blood Lymphocytes of Type 1 Diabetes Patients
Cited by
- Nicotinamide Reduces High Secretion of IFN-γ in High-Risk Relatives Even Though It Does Not Prevent Type 1 Diabetes
- CD8+ T-Cell Responses Identify β-Cell Autoimmunity in Human Type 1 Diabetes
- Combinations of type 1 diabetes, celiac disease and allergy - An immunological challenge
- The impact of harmonization on ELISPOT assay performance.
- Response determination criteria for ELISPOT: toward a standard that can be applied across laboratories.
- The challenge of measuring elusive immune markers by enzyme-linked immuno-spot (ELISPOT) technique.
- Immunotherapies and immune biomarkers in Type 1 diabetes: A partnership for success.
- Association between immune reactivity and disease status of type 1, type 2 diabetes and latent autoimmune diabetes in adults
- Circulating concentrations of cytokines as markers for pathogenic processes in type 1 diabetes and their association with ß-cell function, ß-cell stress and metabolic status
- CD4+CD45RA−FoxP3high activated regulatory T cells are functionally impaired and related to residual insulin‐secreting capacity in patients with type 1 diabetes
- Analysis of Anthrax and Plague Biowarfare Vaccine Interactions with Human Monocyte-Derived Dendritic Cells1
- Current approaches to measuring human islet‐antigen specific T cell function in type 1 diabetes
- Biomarkers for type 1 diabetes.
- Precursor frequencies of T-cells reactive to insulin in recent onset type 1 diabetes mellitus.
- High T‐helper‐1 cytokines but low T‐helper‐3 cytokines, inflammatory cytokines and chemokines in children with high risk of developing type 1 diabetes
- Harmonisation of short-term in vitro culture for the expansion of antigen-specific CD8+ T cells with detection by ELISPOT and HLA-multimer staining
- Expanded T cells from pancreatic lymph nodes of type 1 diabetic subjects recognize an insulin epitope
- Comparison of cryopreservation methods on T‐cell responses to islet and control antigens from type 1 diabetic patients and controls
- CD 127− and FoxP3+ Expression on CD25+CD4+ T Regulatory Cells upon Specific Diabetogeneic Stimulation in High‐risk Relatives of Type 1 Diabetes Mellitus Patients
- Enhancement of cellular memory by reducing stochastic transitions
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