Visualizing Viral Protein Structures in Cells Using Genetic Probes for Correlated Light and Electron Microscopy
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Summary
New methods to visualize viral proteins and structures at high resolutions in-situ using correlated light and electron microscopy (CLEM) are discussed, including the use of genetically encoded protein fusions that oxidize diaminobenzidine into an osmiophilic polymer that can be visualized by EM.
- Type
- review
- Published
- 2015-06-09
- Cited by
- 29
- References
- 71
- Access
- Open access
- OpenAlex
- https://openalex.org/W599500541
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:7631690
Keywords
Viral protein, Biology, Viral structural protein, Cell biology, Viral replication
References
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- Site-specific 3D imaging of cells and tissues with a dual beam microscope.
- FIB/SEM tomography with TEM-like resolution for 3D imaging of high-pressure frozen cells
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE
Cited by
- Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy.
- Applications of genetically encoded photosensitizer miniSOG: from correlative light electron microscopy to immunophotosensitizing
- Shedding light on biology of bacterial cells
- Correlative Light and Electron Microscopy (CLEM) and its applications in infectious disease
- Activation and Utilization of DNA Damage Signaling by Murine Polyomavirus
- ChromEMT: Visualizing 3D chromatin structure and compaction n interphase and mitotic cells
- A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure
- Fluorescent Flavoprotein Heterodimers: Combining Photostability with Singlet Oxygen Generation
- Correlative microscopy for structural microbiology.
- Serial block face scanning electron microscopy in cell biology: Applications and technology.
- An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
- Linhagens celulares derivadas de cultivos primários de neoplasias infectadas pelo BPV como modelo de estudo da transição epitélio-mesênquima.
- Electron tomography of plant organelles and the outlook for correlative microscopic approaches.
- Immunoelectron microscopy of viral antigens
- Imaging the adenovirus infection cycle
- AutoCLEM: An Automated Workflow for Correlative Live-Cell Fluorescence Microscopy and Cryo-Electron Tomography
- Rational design of bioavailable photosensitizers for manipulation and imaging of biological systems
- Growing tool-kit of photosensitizers for clinical and non-clinical applications.
- Chromothripsis drives the evolution of gene amplification in cancer
- Identifying Active Progeny Virus Particles in Formalin-Fixed, Paraffin-Embedded Sections Using Correlative Light and Scanning Electron Microscopy.
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