New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications.
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Summary
Affinities in vitro and detection limits in living cells are optimized with Xaa-Xaa = Pro-Gly, suggesting that the preferred peptide conformation is a hairpin rather than the previously proposed alpha-helix.
- Type
- article
- Published
- 2002-05-02
- Cited by
- 877
- References
- 8
- OpenAlex
- https://openalex.org/W1973562367
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:17170469
Keywords
Chemistry, Peptide, Dissociation constant, Fluorescence, In vitro
References
Cited by
- A modified inverse PCR procedure for insertion, deletion, or replacement of a DNA fragment in a target sequence and its application in the ligand interaction scan method for generation of ligand-regulated proteins.
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- The controlled display of biomolecules on nanoparticles: a challenge suited to bioorthogonal chemistry.
- Application of in vivo cryotechnique to the examination of cells and tissues in living animal organs.
- DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells.
- Intracellular quantitative detection of human thymidylate synthase engagement with an unconventional inhibitor using tetracysteine-diarsenical-probe technology
- Quantitative monitoring of the cytoplasmic release of NCp7 proteins from individual HIV-1 viral cores during the early steps of infection
- Insights into cell wall synthesis and cell division in Staphylococcus aureus
- New sphingolipid probes for metabolism and trafficking studies
- Minimalistic probes for protein folding and structure studies
- Molecular Mechanisms of Endocytosis: Trafficking and Functional Requirements for the Transferrin Receptor, Small Interfering RNAs and Dopamine Transporter: A Dissertation
- Biosensors for ligand detection.
- Protein labeling with FlAsH and ReAsH.
- Single-molecule FRET Studies on DNA Mismatch Repair
- IMAGING FRET: EFFECT OF PROTEIN CONCENTRATION AND COMPARISON OF INTENSITY AND LIFETIME METHODS
- Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis.
- Biophysical studies of peptides with functions in biotechnology and biology
- The Development of New Tools for the Investigation of Protein Function Using Photo-Reactive Unnatural Amino Acids
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