Nonradioactive RNA in situ hybridization detection of human papillomavirus 16-E7 transcripts in squamous cell carcinomas of the uterine cervix using confocal laser scan microscopy.
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Summary
Results indicate continuous expression of E7 ORF in all cervical carcinomas containing HPV 16 DNA and support an active role of the E7 OrF in the pathogenesis of cervical cancer.
- Type
- article
- Published
- 1991-11-01
- Cited by
- 39
- References
- 35
- Access
- Open access
- OpenAlex
- https://openalex.org/W307125185
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:8877123
Keywords
Biology, In situ hybridization, Molecular biology, RNA, Gene expression
References
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- Topography of early HPV 16 transcription in high-grade genital precancers.
- Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers
- Transcriptional differences of the human papillomavirus type 16 genome between precancerous lesions and invasive carcinomas
- Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines
- Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells.
- Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein.
- Structure and transcription of human papillomavirus sequences in cervical carcinoma cells
- A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions.
- Characterization of rare human papillomavirus type 11 mRNAs coding for regulatory and structural proteins, using the polymerase chain reaction.
- In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma.
- The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product.
- Association of human papillomavirus types 16 and 18 E6 proteins with p53.
- Human papillomavirus type 16 alters human epithelial cell differentiation in vitro.
- Potential clinical uses of laser scan microscopy.
- Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product
- Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
Cited by
- PD-1/PD-L1 blockade in cervical cancer: current studies and perspectives
- Detection of human papillomavirus DNA in genital lesions by enzymatic in situ hybridization with Fast Red and laser scanning confocal microscopy
- Uterine cervical neoplasia : aspects of biology and pathology
- Identification of viral infection by confocal microscopy.
- Human papillomavirus type 16 is an important infectious factor in the high incidence of esophageal cancer in Anyang area of China.
- Do HPV‐negative cervical carcinomas exist?—revisited
- RXRα deletion and E6E7 oncogene expression are sufficient to induce cervical malignant lesions in vivo.
- Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization
- Expression of the homeobox gene HOXC4 in keratinocytes of normal skin and epithelial skin tumors is correlated with differentiation.
- Human papillomavirus is a necessary cause of invasive cervical cancer worldwide
- Quantification of biotinylated RNA probes for in situ hybridization using chemiluminescence
- Combined analysis of in situ hybridization, cell cycle and structural markers using reflectance and immunofluorescence confocal microscopy
- Follow‐up of antibody responses to human papillomavirus type 16 E7 in patients treated for cervical carcinoma
- Detection and amplification systems for sensitive, multiple-target DNA and RNA in situ hybridization: looking inside cells with a spectrum of colors
- Multiprimed cDNA synthesis followed by PCR is the most suitable method for Epstein-Barr virus transcript analysis in small lymphoma biopsies.
- Immunosuppressive Tumor Microenvironment in Cervical Cancer Patients
- Prevalence and expression of human papillomavirus in tonsillar carcinomas, indicating a possible viral etiology
- Confocal microscopy in pharmacological research.
- Expression of bcl‐2 protein and transcription of the Epstein‐Barr virus bcl‐2 homologue BHRF‐1 in Hodgkin's disease: implications for different pathogenic mechanisms
- HPV‐mediated cervical carcinogenesis: concepts and clinical implications
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