A post‐ribosomal supernatant from activated Xenopus eggs that displays post‐translationally regulated oscillation of its cdc2+ mitotic kinase activity.
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Summary
A cell‐free extract prepared from activated Xenopus eggs by high‐speed centrifugation displays one spontaneous cycle of activation and inactivation of histone H1 kinase and MPF activity that is largely attributable to Xenopus p32cdc2, suggesting that protein phosphorylation is required for cyclin destruction and kinase inactivation.
- Type
- article
- Published
- 1989-10-01
- Cited by
- 104
- References
- 1
- Access
- Open access
- OpenAlex
- https://openalex.org/W253208710
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:22934599
Keywords
Biology, Xenopus, Cyclin-dependent kinase 1, Molecular biology, Ribosomal protein
References
Cited by
- A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes
- Assay of membrane motility in interphase and metaphase Xenopus extracts.
- Regulation of Cdc2 activity by phosphorylation at T14/Y15.
- Egg extracts for nuclear import and nuclear assembly reactions.
- Protein kinase assays for measuring MPF and MAPK activities in mouse and rat oocytes and early embryos.
- The fenestrin antigen in submembrane skeleton of the ciliate Tetrahymena thermophila is proposed as a marker of cell polarity during cell division and in oral replacement.
- Regulation of the G2-mitosis transition.
- Timing of calcium and protein synthesis requirements for the first mitotic cell cycle in fertilised Xenopus eggs.
- An okadaic acid-sensitive phosphatase negatively controls the cyclin degradation pathway in amphibian eggs
- Calcium requirements during mitotic cdc2 kinase activation and cyclin degradation in Xenopus egg extracts.
- The efficiency and timing of plasmid DNA replication in Xenopus eggs: correlations to the extent of prior chromatin assembly.
- In vivo regulation of MPF in Xenopus oocytes.
- Association of cyclin-bound p34cdc2 with subcellular structures in xenopus eggs.
- Cell cycle modification during the transitions between meiotic M-phases in mouse oocytes.
- The Mre11/Rad50/Nbs1 complex functions in resection-based DNA end joining in Xenopus laevis
- Changes in intracellular pH following egg activation and during the early cell cycle of the amphibian Pleurodeles waltlii, coincide with changes in MPF activity
- A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
- Reactivation of DNA replication in erythrocyte nuclei by Xenopus egg extract involves energy-dependent chromatin decondensation and changes in histone phosphorylation.
- Intracellular free calcium oscillates during cell division of Xenopus embryos
- Microtubule assembly in clarified Xenopus egg extracts.
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