Representative in Vitro cDNA Amplification From Individual Hemopoietic Cells and Colonies
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Summary
The method was applied to small numbers of hemopoietic cells of various lineages, as well as to single micromanipulated cells, and confirmed that the procedure could detect low abundance sequences at least as rare as .025%, and that relative abundance was preserved during amplification.
- Type
- article
- Published
- 1990-01-01
- Cited by
- 213
- References
- 18
- OpenAlex
- https://openalex.org/W70742678
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:39940906
Keywords
Complementary DNA, Molecular biology, Biology, Rapid amplification of cDNA ends, Cloning (programming)
References
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Cited by
- Gene expression in individual cells: analysis using global single cell reverse transcription polymerase chain reaction (GSC RT-PCR).
- Functional and molecular analysis of hematopoietic progenitors derived from the aorta-gonad-mesonephros region of the mouse embryo.
- Growth and differentiation of a bipotent precursor of B cells and macrophages.
- HPK1, a hematopoietic protein kinase activating the SAPK/JNK pathway.
- Recombinant human interleukin-11 synergizes with steel factor and interleukin-3 to promote directly the early stages of murine megakaryocyte development in vitro.
- An endodarwinian approach of intercellular variability of gene expression
- Unicellular-unilineage erythropoietic cultures: molecular analysis of regulatory gene expression at sibling cell level.
- Rapid upregulation of fibroblast growth factor receptor 1 (flg) by rat photoreceptor cells after injury.
- Single‐cell gene profiling of planarian stem cells using fluorescent activated cell sorting and its “index sorting” function for stem cell research
- Fusarium oxysporum from iridaceous crops: analysis of genetic diversity and host specialisation
- Techniques for the study of adult stem cells: be fruitful and multiply.
- Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling
- Application of an RNA amplification method for reliable single-cell transcriptome analysis
- Evaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells
- Development of erythroid and myeloid progenitors in the yolk sac and embryo proper of the mouse.
- Clonal analysis of differentiating embryonic stem cells reveals a hematopoietic progenitor with primitive erythroid and adult lymphoid-myeloid potential.
- Evidence for Diversity in Transcriptional Profiles of Single Hematopoietic Stem Cells
- Comparative Expression of a Set of Genes to an Internal Housekeeping Control in CDNA Amplified and not Amplified by PolyAPCR in Non-Hodgkin's Lymphoma Samples Obtained From Fine-Needle Aspiration Cytology
- Analysis of G protein α subunit mRNA abundance in preimplantation mouse embryos using a rapid, quantitative RT‐PCR approach
- Analysis of gene expression in a complex differentiation hierarchy by global amplification of cDNA from single cells.
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