Using LacO arrays to monitor DNA double-strand break dynamics in live Schizosaccharomyces pombe cells.
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Summary
A novel approach is developed, based on the principles used for homologous recombination-based genome engineering in higher eukaryotes, to integrate long, repetitive LacO arrays with targeting efficiencies as high as 70 % in S. pombe.
- Type
- article
- Published
- 2014-01-01
- Cited by
- 13
- References
- 32
- OpenAlex
- https://openalex.org/W25030924
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10935938
Keywords
Computer science
References
- Genomic instability — an evolving hallmark of cancer
- Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual
- Methods in Yeast Genetics: A Laboratory Course Manual
- A new method to efficiently induce a site-specific double strand break in the fission yeast Schizosaccharomyces pombe
- GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion.
- New Tool for Genome Surgery
- Heterologous modules for efficient and versatile PCR‐based gene targeting in Schizosaccharomyces pombe
- Functional Targeting of DNA Damage to a Nuclear Pore–Associated SUMO-Dependent Ubiquitin Ligase
- Chromatin movement in the maintenance of genome stability.
- Visualizing chromosome dynamics with GFP.
- Mating-Type Genes and MAT Switching in Saccharomyces cerevisiae
- ATM signaling facilitates repair of DNA double-strand breaks associated with heterochromatin.
- The double-strand-break repair model for recombination.
- Recruitment of the INO80 complex by H2A phosphorylation links ATP-dependent chromatin remodeling with DNA double-strand break repair.
- The Fun30 ATP-dependent nucleosome remodeler promotes resection of DNA double-strand break ends
- TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility.
- Double-Strand Breaks in Heterochromatin Move Outside of a Dynamic HP1a Domain to Complete Recombinational Repair
- Modules for cloning‐free chromatin tagging in Saccharomyces cerevisae
- Regulation of gene expression at the fission yeast Schizosaccharomyces pombe urg1 locus.
- Size and number of tandem repeat arrays can determine somatic homologous pairing of transgene loci mediated by epigenetic modifications in Arabidopsis thaliana nuclei
Cited by
- Studies on regulation of the cell cycle in fission yeast.
- Improved Determination of Subnuclear Position Enabled by Three-Dimensional Membrane Reconstruction.
- Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks
- Schizosaccharomyces pombe Assays to Study Mitotic Recombination Outcomes
- Homology-directed repair involves multiple strand invasion cycles in fission yeast
- Covariance distributions in single particle tracking
- Chromatin dynamics are constrained by loops and driven by the INO80 nucleosome remodeler
- Loops and the activity of loop extrusion factors constrain chromatin dynamics
- SUMO in the regulation of DNA repair and transcription at nuclear pores
- Coarse-grained chromatin dynamics by tracking multiple similarly labeled gene loci
- The LINC complex component Kms1 and CENP-B protein Cbp1 cooperate to enforce faithful homology-directed DNA repair at the nuclear periphery in S. pombe
- The LINC complex component Kms1 and CENP-B protein Cbp1 cooperate to enforce faithful homology-directed DNA repair at the nuclear periphery in S. pombe
- Chromatin dynamics are constrained by loops and driven by the INO80 nucleosome remodeler
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