The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation
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Summary
The simultaneous measurement of precise and accurate 3D single-molecule position and 3D dipole orientation using the Double-Helix Point Spread Function (DH-PSF) microscope is reported, able to significantly improve dipole-induced position errors.
- Type
- article
- Published
- 2013-02-22
- Cited by
- 33
- References
- 27
- Access
- Open access
- OpenAlex
- https://openalex.org/W24817798
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:34934467
Keywords
Political science
References
- Microscopy beyond the diffraction limit using actively controlled single molecules
- SIMULTANEOUS IMAGING OF INDIVIDUAL MOLECULES ALIGNED BOTH PARALLEL AND PERPENDICULAR TO THE OPTIC AXIS
- Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples
- Double helix PSF engineering for computational fluorescence microscopy imaging
- Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
- Microscopy and its focal switch
- The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision.
- Simultaneous, accurate measurement of the 3D position and orientation of single molecules
- Rotational Mobility of Single Molecules Affects Localization Accuracy in Super-Resolution Fluorescence Microscopy
- Polarization effect on position accuracy of fluorophore localization.
- Photophysical properties of acene DCDHF fluorophores: long-wavelength single-molecule emitters designed for cellular imaging.
- Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
- Optimized localization-analysis for single-molecule tracking and super-resolution microscopy
- Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.
- Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.
- Orientation imaging of single molecules by wide-field epifluorescence microscopy
- Position and orientation estimation of fixed dipole emitters using an effective Hermite point spread function model.
- Molecular orientation affects localization accuracy in superresolution far-field fluorescence microscopy.
- Single-molecule orientations determined by direct emission pattern imaging
- Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy
Cited by
- Aberration influenced generation of rotating two-lobe light fields
- Single Particle Tracking: From Theory to Biophysical Applications.
- Computational Imaging using Electromagnetic Optics
- Rotation of two-petal laser beams in the near field of a spiral microaxicon
- Simulation of the Optical Vortices Generation by the Silver Spiral Zone Plates
- Amplitude spiral zone plates for generation of optical vortices
- Two-petal laser beam near a binary spiral axicon with topological charge 2
- The Backflow of Energy in a Optical Vortex Formed by Silver Spiral Zone Plate
- Waveguide platform and methods for super-resolution fluorescence microscopy of sub-cellular structures
- Orbital angular momentum of generalized cosine Gaussian beams with an infinite number of screw dislocations
- Propagation-invariant laser beams with an array of phase singularities
- Propagation-Invariant Off-Axis Elliptic Gaussian Beams with the Orbital Angular Momentum
- Comparative modeling of optical vortex focusing by Fresnel zone plates
- Recent progress on single-molecule localization microscopy
- Off-axis elliptic Gaussian beams with an intrinsic orbital angular momentum
- Paraxial shifted elliptic beams, conserving their shape on free space propagation
- Diffraction of laser radiation by a binary zone plate with fractional order
- Comparison of the shape of focal spots in terms of intensity and energy flux for a high-aperture zone plate and a spiral zone plate
- New type of vortex laser beams: squared Laguerre-Gauss beam
- Propagation-Invariant Off-Axis Elliptical Beams and their Orbital Angular Momentum
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